PMID- 9020085
OWN - NLM
STAT- MEDLINE
DCOM- 19970310
LR  - 20190618
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 275
IP  - 5302
DP  - 1997 Feb 14
TI  - Regulation of replicon size in Xenopus egg extracts.
PG  - 993-5
AB  - Once a specific number of cells have been produced in the early Xenopus laevis 
      embryo, replicon size during the S phase of the cell cycle increases. Here, it is 
      reported that similar increase in replicon size occurred when the concentration 
      of nuclei in replication-competent Xenopus egg extracts exceeded a critical 
      threshold. In this system, the origin recognition complex (ORC) did not become 
      stoichiometrically limiting for initiation, and similar amounts of this complex 
      bound to chromatin regardless of replicon size. These data suggest that in early 
      development, an unidentified factor controls how many preformed ORC-DNA complexes 
      initiate DNA replication.
FAU - Walter, J
AU  - Walter J
AD  - Department of Biology, University of California, San Diego, La Jolla, CA 
      92093-0347, USA.
FAU - Newport, J W
AU  - Newport JW
LA  - eng
GR  - 1F32FM17980-01/PHS HHS/United States
GR  - R01FM44656/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chromatin)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Cyclins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Origin Recognition Complex)
RN  - 04079A1RDZ (Cytarabine)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cell Cycle Proteins/metabolism
MH  - Chromatin/metabolism
MH  - Cyclin-Dependent Kinase Inhibitor p21
MH  - Cyclins/pharmacology
MH  - Cytarabine/pharmacology
MH  - DNA/*metabolism
MH  - *DNA Replication
MH  - DNA-Binding Proteins/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Origin Recognition Complex
MH  - Ovum/*metabolism
MH  - Replication Origin
MH  - *Replicon
MH  - S Phase
MH  - Xenopus laevis
EDAT- 1997/02/14 00:00
MHDA- 1997/02/14 00:01
CRDT- 1997/02/14 00:00
PHST- 1997/02/14 00:00 [pubmed]
PHST- 1997/02/14 00:01 [medline]
PHST- 1997/02/14 00:00 [entrez]
AID - 10.1126/science.275.5302.993 [doi]
PST - ppublish
SO  - Science. 1997 Feb 14;275(5302):993-5. doi: 10.1126/science.275.5302.993.