PMID- 9034148
OWN - NLM
STAT- MEDLINE
DCOM- 19970314
LR  - 20240213
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 185
IP  - 4
DP  - 1997 Feb 17
TI  - The immunodominant antigen of an ultraviolet-induced regressor tumor is generated 
      by a somatic point mutation in the DEAD box helicase p68.
PG  - 695-705
AB  - The genetic origins of CD8+ T cell-recognized unique antigens to which mice 
      respond when immunized with syngeneic tumor cells are unknown. The ultraviolet 
      light-induced murine tumor 8101 expresses an H-2Kb-restricted immunodominant 
      antigen, A, that induces cytolytic CD8+ T cells in vivo A+ 8101 cells are 
      rejected by naive mice while A- 8101 tumor cells grow. To identify the antigen 
      H-2Kb molecules were immunoprecipitated from A+ 8101 cells and peptides were 
      eluted by acid. The sensitizing peptide was isolated by sequential reverse-phase 
      HPLC and sequenced using microcapillary HPLC-triple quadruple mass spectrometry. 
      The peptide, SNFVFAGI, matched the sequence of the DEAD box protein p68 RNA 
      helicase except for a single amino acid substitution, caused by a single 
      nucleotide change. This mutation was somatic since fibroblasts from the mouse of 
      tumor origin expressed the wild-type sequence. The amino acid substitution 
      created an anchor for binding of the mutant peptide to H-2Kb. Our results are 
      consistent with mutant p68 being responsible for rejection of the tumor. Several 
      functions of p68, which include nucleolar assembly and inhibition of DNA 
      unwinding, may be mediated through its IQ domain, which was altered by the 
      mutation. This is the first description of a somatic tumor-specific mutation in 
      the coding region of a nucleic acid helicase.
FAU - Dubey, P
AU  - Dubey P
AD  - Department of Pathology, The University of Chicago, Illinois 60637, USA.
FAU - Hendrickson, R C
AU  - Hendrickson RC
FAU - Meredith, S C
AU  - Meredith SC
FAU - Siegel, C T
AU  - Siegel CT
FAU - Shabanowitz, J
AU  - Shabanowitz J
FAU - Skipper, J C
AU  - Skipper JC
FAU - Engelhard, V H
AU  - Engelhard VH
FAU - Hunt, D F
AU  - Hunt DF
FAU - Schreiber, H
AU  - Schreiber H
LA  - eng
GR  - R01CA37156/CA/NCI NIH HHS/United States
GR  - R01 CA037156/CA/NCI NIH HHS/United States
GR  - P01 CA019266/CA/NCI NIH HHS/United States
GR  - R01 AI033993/AI/NIAID NIH HHS/United States
GR  - R01 AI020963/AI/NIAID NIH HHS/United States
GR  - R01CA22677/CA/NCI NIH HHS/United States
GR  - R01CA19266/CA/NCI NIH HHS/United States
GR  - R01 CA022677/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens)
RN  - 0 (DNA, Complementary)
RN  - 0 (H-2 Antigens)
RN  - 0 (H-2Kb protein, mouse)
RN  - 0 (Immunodominant Epitopes)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.7.- (RNA Nucleotidyltransferases)
RN  - EC 3.6.1.- (Ddx5 protein, mouse)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
RN  - EC 3.6.4.13 (RNA Helicases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens/*immunology
MH  - Base Sequence
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - DEAD-box RNA Helicases
MH  - DNA, Complementary
MH  - Female
MH  - H-2 Antigens/immunology
MH  - Immunodominant Epitopes/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Neoplasms, Experimental/immunology
MH  - *Point Mutation
MH  - *Protein Kinases
MH  - *RNA Helicases
MH  - RNA Nucleotidyltransferases/*genetics
PMC - PMC2196148
EDAT- 1997/02/17 00:00
MHDA- 1997/02/17 00:01
PMCR- 1997/08/17
CRDT- 1997/02/17 00:00
PHST- 1997/02/17 00:00 [pubmed]
PHST- 1997/02/17 00:01 [medline]
PHST- 1997/02/17 00:00 [entrez]
PHST- 1997/08/17 00:00 [pmc-release]
AID - 10.1084/jem.185.4.695 [doi]
PST - ppublish
SO  - J Exp Med. 1997 Feb 17;185(4):695-705. doi: 10.1084/jem.185.4.695.