PMID- 9053841
OWN - NLM
STAT- MEDLINE
DCOM- 19970307
LR  - 20220310
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 14
IP  - 4
DP  - 1997 Jan 30
TI  - Molecular characterization of ALK, a receptor tyrosine kinase expressed 
      specifically in the nervous system.
PG  - 439-49
AB  - The 2;5 chromosomal translocation is frequently associated with anaplastic large 
      cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the 
      alk (anaplastic lymphoma kinase) gene and the nucelophosmin (npm) gene: the 3' 
      half of alk derived from chromosome 2 is fused to the 5' portion of npm from 
      chromosome 5. A recent study shows that the product of the npm-alk fusion gene is 
      oncogenic. To help understand how the npm-alk oncogene transform cells, it is 
      important to investigate the normal biological function of the alk gene product, 
      ALK. Here, we show molecular cloning of cDNAs for both the human and mouse ALK 
      proteins. The deduced amino acid sequences reveal that ALK is a novel receptor 
      protein-tyrosine kinase having a putative transmembrane domain and an 
      extracellular domain. These sequences are absent in the product of the 
      transforming npm-alk gene. ALK shows the greatest sequence similarity to LTK 
      (leukocyte tyrosine kinase) whose biological function is presently unknown. RNA 
      blot hybridization analysis of various tissues reveals that the alk mRNA is 
      dominantly detected in the brain and spinal cord. Immunoblotting with anti-ALK 
      antibody shows that ALK is highly expressed in the neonatal brain. Furthermore, 
      RNA in situ hybridization analysis shows that the alk mRNA is dominantly 
      expressed in neurons in specific regions of the nervous system such as the 
      thalamus, mid-brain, olfactory bulb, and ganglia of embryonic and neonatal mice. 
      These data suggest that ALK plays an important role(s) in the development of the 
      brain and exerts its effects on specific neurons in the nervous system.
FAU - Iwahara, T
AU  - Iwahara T
AD  - Department of Oncology, Institute of Medical Science, University of Tokyo, Japan.
FAU - Fujimoto, J
AU  - Fujimoto J
FAU - Wen, D
AU  - Wen D
FAU - Cupples, R
AU  - Cupples R
FAU - Bucay, N
AU  - Bucay N
FAU - Arakawa, T
AU  - Arakawa T
FAU - Mori, S
AU  - Mori S
FAU - Ratzkin, B
AU  - Ratzkin B
FAU - Yamamoto, T
AU  - Yamamoto T
LA  - eng
SI  - GENBANK/D83002
SI  - GENBANK/U66559
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA, Complementary)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 117896-08-9 (Nucleophosmin)
RN  - EC 2.7.10.1 (ALK protein, human)
RN  - EC 2.7.10.1 (Alk protein, mouse)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Anaplastic Lymphoma Kinase
MH  - Animals
MH  - Animals, Newborn
MH  - Base Sequence
MH  - Brain/embryology/*metabolism
MH  - Chromosomes, Human, Pair 2
MH  - Chromosomes, Human, Pair 5
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Embryo, Mammalian
MH  - Ganglia/embryology/metabolism
MH  - Gene Expression
MH  - Gene Library
MH  - Humans
MH  - In Situ Hybridization
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neurons/*metabolism
MH  - Nuclear Proteins/biosynthesis/genetics
MH  - Nucleophosmin
MH  - Organ Specificity
MH  - Protein-Tyrosine Kinases/*biosynthesis/chemistry/*genetics
MH  - Receptor Protein-Tyrosine Kinases
MH  - Recombinant Fusion Proteins/biosynthesis/chemistry
MH  - Sequence Homology, Amino Acid
MH  - Spinal Cord/embryology/*metabolism
MH  - Testis/metabolism
MH  - Translocation, Genetic
EDAT- 1997/01/30 00:00
MHDA- 1997/01/30 00:01
CRDT- 1997/01/30 00:00
PHST- 1997/01/30 00:00 [pubmed]
PHST- 1997/01/30 00:01 [medline]
PHST- 1997/01/30 00:00 [entrez]
AID - 10.1038/sj.onc.1200849 [doi]
PST - ppublish
SO  - Oncogene. 1997 Jan 30;14(4):439-49. doi: 10.1038/sj.onc.1200849.