PMID- 9065401
OWN - NLM
STAT- MEDLINE
DCOM- 19970422
LR  - 20240104
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 275
IP  - 5307
DP  - 1997 Mar 21
TI  - Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC-/- 
      colon carcinoma.
PG  - 1784-7
AB  - The adenomatous polyposis coli (APC) tumor suppressor protein binds to 
      beta-catenin, a protein recently shown to interact with Tcf and Lef transcription 
      factors. The gene encoding hTcf-4, a Tcf family member that is expressed in 
      colonic epithelium, was cloned and characterized. hTcf-4 transactivates 
      transcription only when associated with beta-catenin. Nuclei of APC-/- colon 
      carcinoma cells were found to contain a stable beta-catenin-hTcf-4 complex that 
      was constitutively active, as measured by transcription of a Tcf reporter gene. 
      Reintroduction of APC removed beta-catenin from hTcf-4 and abrogated the 
      transcriptional transactivation. Constitutive transcription of Tcf target genes, 
      caused by loss of APC function, may be a crucial event in the early 
      transformation of colonic epithelium.
FAU - Korinek, V
AU  - Korinek V
AD  - Department of Immunology, University Hospital, Post Office Box 85500, 3508 GA 
      Utrecht, Netherlands.
FAU - Barker, N
AU  - Barker N
FAU - Morin, P J
AU  - Morin PJ
FAU - van Wichen, D
AU  - van Wichen D
FAU - de Weger, R
AU  - de Weger R
FAU - Kinzler, K W
AU  - Kinzler KW
FAU - Vogelstein, B
AU  - Vogelstein B
FAU - Clevers, H
AU  - Clevers H
LA  - eng
SI  - GENBANK/Y11306
GR  - CA57345/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Adenomatous Polyposis Coli Protein)
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (CTNNB1 protein, mouse)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (TCF Transcription Factors)
RN  - 0 (TCF7L2 protein, human)
RN  - 0 (Tcf7l2 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factor 7-Like 2 Protein)
RN  - 0 (Transcription Factors)
RN  - 0 (beta Catenin)
SB  - IM
CIN - Science. 1997 Mar 21;275(5307):1752-3. PMID: 9122680
MH  - Adenomatous Polyposis Coli Protein
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cell Transformation, Neoplastic
MH  - Cloning, Molecular
MH  - Colon/metabolism
MH  - Colonic Neoplasms/*genetics/metabolism
MH  - Cytoskeletal Proteins/genetics/*metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - *Genes, APC
MH  - Genes, Reporter
MH  - Humans
MH  - Intestinal Mucosa/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Signal Transduction
MH  - TCF Transcription Factors
MH  - *Trans-Activators
MH  - Transcription Factor 7-Like 2 Protein
MH  - Transcription Factors/chemistry/genetics/*metabolism
MH  - *Transcriptional Activation
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - beta Catenin
EDAT- 1997/03/21 00:00
MHDA- 1997/03/21 00:01
CRDT- 1997/03/21 00:00
PHST- 1997/03/21 00:00 [pubmed]
PHST- 1997/03/21 00:01 [medline]
PHST- 1997/03/21 00:00 [entrez]
AID - 10.1126/science.275.5307.1784 [doi]
PST - ppublish
SO  - Science. 1997 Mar 21;275(5307):1784-7. doi: 10.1126/science.275.5307.1784.