PMID- 9086446
OWN - NLM
STAT- MEDLINE
DCOM- 19970626
LR  - 20191101
IS  - 1073-9688 (Print)
IS  - 1073-9688 (Linking)
VI  - 3
IP  - 4
DP  - 1996 Dec
TI  - Perfusion of single tumor microvessels: application to vascular permeability 
      measurement.
PG  - 349-57
AB  - OBJECTIVE: To develop a new method for determining the relative importance of 
      convection versus diffusion in macromolecular transport across tumor microvessel 
      walls. METHODS: The human colon adenocarcinoma LS174T was transplanted in the 
      dorsal skinfold chamber in a severe combined immunodeficient (SCID) mouse. The 
      vasculature at the tumor surface was exposed by carefully removing the glass 
      window of the chamber. A tumor microvessel was randomly selected, which was 
      approximately 20-40 microns in diameter, embedded in the connective tissue 10-12 
      microns below the surface of the tumor. The vessel was cannulated with a 
      micropipette and perfused with fluorescein isothiocyanate (FITC)-labeled bovine 
      serum albumin (BSA) at different perfusion pressures. The fluorescence intensity 
      was recorded on videotapes via a video system attached to the fluorescence 
      microscope for offline analysis. The apparent vascular permeability was 
      determined based on the time-dependence of fluorescence intensity and the vessel 
      diameter. RESULTS: The apparent vascular permeability of single vessels to 
      FITC-labeled BSA was quantified at perfusion pressures of 20-45 cmH2O. The 
      pressure dependence of vascular permeability in LS174T tumors was heterogeneous. 
      On average, there was no correlation between the apparent vascular permeability 
      and the perfusion pressure in the range of 20-35 cmH2O (p = 0.73), even though 
      the apparent permeability increased significantly when the pressure was increased 
      from 20 to 45 cmH2O (p = 0.008). CONCLUSIONS: These results indicate that 
      convection in the transvascular transport of albumin is not significant in 
      non-peripheral regions of solid tumors in which the pressure difference across 
      the vessel wall is small or even negligible. In addition to the permeability 
      studies, this preparation can be used to study cell-cell interactions in single 
      tumor vessels under defined flow conditions.
FAU - Lichtenbeld, H C
AU  - Lichtenbeld HC
AD  - Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical 
      School, Boston 02114, USA.
FAU - Yuan, F
AU  - Yuan F
FAU - Michel, C C
AU  - Michel CC
FAU - Jain, R K
AU  - Jain RK
LA  - eng
GR  - R35-CA56591/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Microcirculation
JT  - Microcirculation (New York, N.Y. : 1994)
JID - 9434935
RN  - 0 (fluorescein isothiocyanate bovine serum albumin)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Adenocarcinoma/*blood supply
MH  - Animals
MH  - Blood Pressure
MH  - Capillary Permeability/*physiology
MH  - Catheterization
MH  - Fluorescein-5-isothiocyanate/analogs & derivatives/pharmacokinetics
MH  - Humans
MH  - Kinetics
MH  - Mice
MH  - Mice, SCID
MH  - Microcirculation/*physiology
MH  - Microscopy, Fluorescence
MH  - Microscopy, Video
MH  - Neoplasm Transplantation
MH  - Perfusion
MH  - Serum Albumin, Bovine/pharmacokinetics
MH  - Transplantation, Heterologous
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - 10.3109/10739689609148307 [doi]
PST - ppublish
SO  - Microcirculation. 1996 Dec;3(4):349-57. doi: 10.3109/10739689609148307.