PMID- 9090378
OWN - NLM
STAT- MEDLINE
DCOM- 19970425
LR  - 20071114
IS  - 1061-4036 (Print)
IS  - 1061-4036 (Linking)
VI  - 15
IP  - 4
DP  - 1997 Apr
TI  - Formation of de novo centromeres and construction of first-generation human 
      artificial microchromosomes.
PG  - 345-55
AB  - We have combined long synthetic arrays of alpha satellite DNA with telomeric DNA 
      and genomic DNA to generate artificial chromosomes in human HT1080 cells. The 
      resulting linear microchromosomes contain exogenous alpha satellite DNA, are 
      mitotically and cytogenetically stable in the absence of selection for up to six 
      months in culture, bind centromere proteins specific for active centromeres, and 
      are estimated to be 6-10 megabases in size, approximately one-fifth to one-tenth 
      the size of endogenous human chromosomes. We conclude that this strategy results 
      in the formation of de novo centromere activity and that the microchromosomes so 
      generated contain all of the sequence elements required for stable mitotic 
      chromosome segregation and maintenance. This first-generation system for the 
      construction of human artificial chromosomes should be suitable for dissecting 
      the sequence requirements of human centromeres, as well as developing constructs 
      useful for therapeutic applications.
FAU - Harrington, J J
AU  - Harrington JJ
AD  - Department of Genetics and Center for Human Genetics, Case Western Reserve 
      University School of Medicine, Cleveland, Ohio, USA.
FAU - Van Bokkelen, G
AU  - Van Bokkelen G
FAU - Mays, R W
AU  - Mays RW
FAU - Gustashaw, K
AU  - Gustashaw K
FAU - Willard, H F
AU  - Willard HF
LA  - eng
GR  - HG00107/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Genet
JT  - Nature genetics
JID - 9216904
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA, Satellite)
RN  - 0 (centromere protein C)
RN  - 0 (centromere protein E)
SB  - IM
CIN - Nat Genet. 1997 Apr;15(4):333-5. PMID: 9090375
MH  - Centromere/genetics
MH  - Chromosomal Proteins, Non-Histone/analysis
MH  - Chromosomes, Human/*genetics
MH  - DNA, Satellite/*genetics
MH  - Fibrosarcoma
MH  - Genetic Vectors/*genetics
MH  - Humans
MH  - Telomere/genetics
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
AID - 10.1038/ng0497-345 [doi]
PST - ppublish
SO  - Nat Genet. 1997 Apr;15(4):345-55. doi: 10.1038/ng0497-345.