PMID- 9096395
OWN - NLM
STAT- MEDLINE
DCOM- 19970508
LR  - 20240214
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 94
IP  - 7
DP  - 1997 Apr 1
TI  - Human cytomagalovirus IE1 and IE2 proteins are mutagenic and mediate 
      "hit-and-run" oncogenic transformation in cooperation with the adenovirus E1A 
      proteins.
PG  - 3341-5
AB  - Some epidemiological studies have suggested a possible link between human 
      cytomegalovirus (HCMV) infection and various malignancies, and HCMV has been 
      shown to transform cultured cells. However, viral DNA is not detected in most 
      transformants, and the mechanism by which HCMV might contribute to oncogenesis 
      has remained obscure. Here we show that the HCMV immediate early 1 and 2 genes 
      can cooperate with the adenovirus E1A gene to generate transformed foci of 
      primary baby rat kidney cells. HCMV gene expression is transient and viral DNA is 
      not present in clonal cell lines derived from the transformed foci. We find that 
      the HCMV immediate early proteins are mutagenic, and we propose that HCMV has the 
      potential to contribute to oncogenesis through a "hit-and-run" mechanism, by 
      inducing mutations in cellular genes.
FAU - Shen, Y
AU  - Shen Y
AD  - Department of Molecular Biology, Princeton University, NJ 08544-1014, USA.
FAU - Zhu, H
AU  - Zhu H
FAU - Shenk, T
AU  - Shenk T
LA  - eng
GR  - P01 CA041086/CA/NCI NIH HHS/United States
GR  - CA41086/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Adenovirus E1A Proteins)
RN  - 0 (IE1 protein, cytomegalovirus)
RN  - 0 (IE2 protein, Cytomegalovirus)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Trans-Activators)
RN  - 0 (UL115 protein, Human herpesvirus 5)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (glycoprotein H, Cytomegalovirus)
RN  - 0 (glycoprotein H, Human cytomegalovirus)
RN  - 0 (glycoprotein O, cytomegalovirus)
SB  - IM
MH  - Adenovirus E1A Proteins/*physiology
MH  - Animals
MH  - CHO Cells
MH  - *Cell Transformation, Neoplastic
MH  - *Cell Transformation, Viral
MH  - Cells, Cultured
MH  - Cricetinae
MH  - Immediate-Early Proteins/*physiology
MH  - *Membrane Glycoproteins
MH  - *Mutagenesis
MH  - Rats
MH  - *Trans-Activators
MH  - *Viral Envelope Proteins
MH  - *Viral Proteins
PMC - PMC20371
EDAT- 1997/04/01 00:00
MHDA- 1997/04/01 00:01
PMCR- 1997/10/01
CRDT- 1997/04/01 00:00
PHST- 1997/04/01 00:00 [pubmed]
PHST- 1997/04/01 00:01 [medline]
PHST- 1997/04/01 00:00 [entrez]
PHST- 1997/10/01 00:00 [pmc-release]
AID - 3944 [pii]
AID - 10.1073/pnas.94.7.3341 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3341-5. doi: 10.1073/pnas.94.7.3341.