PMID- 9129012
OWN - NLM
STAT- MEDLINE
DCOM- 19970603
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 89
IP  - 9
DP  - 1997 May 1
TI  - Lack of expression of Thy-1 (CD90) on acute myeloid leukemia cells with long-term 
      proliferative ability in vitro and in vivo.
PG  - 3104-12
AB  - Acute myeloid leukaemia (AML) is thought to be maintained by a small population 
      of leukemic progenitor cells. To define the phenotype of such cells with 
      long-term proliferative capacity in vitro and in vivo, we have used the 
      production of leukemic clonogenic cells (CFU) after 2 to 8 weeks in suspension 
      culture as a measure of these cells in vitro and compared their phenotype with 
      that of cells capable of engrafting nonobese diabetic severe combined immune 
      deficient (NOD/SCID) mice. Leukemic blast peripheral blood cells were evaluated 
      for expression of CD34 and Thy-1 (CD90) antigens. The majority of AML blast cells 
      at diagnosis lacked expression of Thy-1. Most primary CFU-blast and the CFU 
      detected at up to 8 weeks from suspension cultures were CD34+/Thy-1-. AML cells 
      that were capable of engrafting NOD/SCID mice were also found to have the 
      CD34+/Thy-1- phenotype. However, significant engraftment was achieved using both 
      CD34+/Thy-1- and CD34- subfractions from one AML M5 patient. These results 
      suggest that while heterogeneity exists between individual patients, the leukemic 
      progenitor cells that are capable of maintaining the disease in vitro and in vivo 
      differ from normal hematopoietic progenitor cells in their lack of expression of 
      Thy-1.
FAU - Blair, A
AU  - Blair A
AD  - Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver Hospital and 
      Health Sciences Centre, Canada.
FAU - Hogge, D E
AU  - Hogge DE
FAU - Ailles, L E
AU  - Ailles LE
FAU - Lansdorp, P M
AU  - Lansdorp PM
FAU - Sutherland, H J
AU  - Sutherland HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (Thy-1 Antigens)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*biosynthesis/blood
MH  - Antigens, CD34/blood
MH  - Blast Crisis
MH  - Cell Division
MH  - Flow Cytometry
MH  - Humans
MH  - Leukemia, Myeloid, Acute/*blood/genetics/immunology/pathology
MH  - Leukocyte Count
MH  - Lymphocyte Subsets/immunology
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Thy-1 Antigens/*biosynthesis/blood
MH  - Transplantation, Heterologous
MH  - Tumor Cells, Cultured
MH  - Tumor Stem Cell Assay
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
AID - S0006-4971(20)58320-1 [pii]
PST - ppublish
SO  - Blood. 1997 May 1;89(9):3104-12.