PMID- 9136014
OWN - NLM
STAT- MEDLINE
DCOM- 19970903
LR  - 20210915
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 146
IP  - 1
DP  - 1997 May
TI  - Isolation of mutations in the Drosophila homologues of the human 
      Neurofibromatosis 2 and yeast CDC42 genes using a simple and efficient 
      reverse-genetic method.
PG  - 245-52
AB  - Reverse genetic analysis in Drosophila has been greatly aided by a growing 
      collection of lethal P transposable element insertions that provide molecular 
      tags for the identification of essential genetic loci. However, because the 
      screens performed to date primarily have generated autosomal P-element 
      insertions, this collection has not been as useful for performing reverse genetic 
      analysis of X-linked genes. We have designed a reverse genetic screen that takes 
      advantage of the hemizygosity of the X chromosome in males together with a 
      cosmid-based transgene that serves as an autosomally linked duplication of a 
      small region of the X chromosome. The efficacy and efficiency of this method is 
      demonstrated by the isolation of mutations in Drosophila homologues of two 
      well-studied genes, the human Neurofibromatosis 2 tumor suppressor and the yeast 
      CDC42 gene. The method we describe should be of general utility for the isolation 
      of mutations in other X-linked genes, and should also provide an efficient method 
      for the isolation of new allcles of existing X-linked or autosomal mutations in 
      Drosophila.
FAU - Fehon, R G
AU  - Fehon RG
AD  - Department of Zoology, Duke University, Durham, North Carolina 27708-1000, USA. 
      rfehon@acpub.duke.edu
FAU - Oren, T
AU  - Oren T
FAU - LaJeunesse, D R
AU  - LaJeunesse DR
FAU - Melby, T E
AU  - Melby TE
FAU - McCartney, B M
AU  - McCartney BM
LA  - eng
SI  - GENBANK/U85024
SI  - GENBANK/U85025
SI  - GENBANK/U85026
SI  - GENBANK/U85027
SI  - GENBANK/U85028
SI  - GENBANK/U85029
SI  - GENBANK/U85030
SI  - GENBANK/U85031
SI  - GENBANK/U85032
SI  - GENBANK/U85033
SI  - GENBANK/U85034
GR  - NS-34783/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neurofibromin 2)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (cdc42 GTP-Binding Protein, Saccharomyces cerevisiae)
SB  - IM
MH  - Animals
MH  - Cell Cycle Proteins/*genetics
MH  - Cosmids
MH  - Drosophila/*genetics
MH  - Female
MH  - GTP-Binding Proteins/*genetics
MH  - *Genes, Neurofibromatosis 2
MH  - Genetic Complementation Test
MH  - Humans
MH  - Male
MH  - Membrane Proteins/genetics
MH  - Molecular Sequence Data
MH  - Mutagenesis, Insertional
MH  - Neurofibromin 2
MH  - cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
PMC - PMC1207939
EDAT- 1997/05/01 00:00
MHDA- 1997/05/01 00:01
PMCR- 1997/08/01
CRDT- 1997/05/01 00:00
PHST- 1997/05/01 00:00 [pubmed]
PHST- 1997/05/01 00:01 [medline]
PHST- 1997/05/01 00:00 [entrez]
PHST- 1997/08/01 00:00 [pmc-release]
AID - 1461245 [pii]
AID - 10.1093/genetics/146.1.245 [doi]
PST - ppublish
SO  - Genetics. 1997 May;146(1):245-52. doi: 10.1093/genetics/146.1.245.