PMID- 9144202
OWN - NLM
STAT- MEDLINE
DCOM- 19970605
LR  - 20190524
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 94
IP  - 10
DP  - 1997 May 13
TI  - Cooperative functions of the reaper and head involution defective genes in the 
      programmed cell death of Drosophila central nervous system midline cells.
PG  - 5131-6
AB  - In Drosophila, the chromosomal region 75C1-2 contains at least three genes, 
      reaper (rpr), head involution defective (hid), and grim, that have important 
      functions in the activation of programmed cell death. To better understand how 
      cells are killed by these genes, we have utilized a well defined set of embryonic 
      central nervous system midline cells that normally exhibit a specific pattern of 
      glial cell death. In this study we show that both rpr and hid are expressed in 
      dying midline cells and that the normal pattern of midline cell death requires 
      the function of multiple genes in the 75C1-2 interval. We also utilized the 
      P[UAS]/P[Gal4] system to target expression of rpr and hid to midline cells. 
      Targeted expression of rpr or hid alone was not sufficient to induce ectopic 
      midline cell death. However, expression of both rpr and hid together rapidly 
      induced ectopic midline cell death that resulted in axon scaffold defects 
      characteristic of mutants with abnormal midline cell development. 
      Midline-targeted expression of the baculovirus p35 protein, a caspase inhibitor, 
      blocked both normal and ectopic rpr- and hid-induced cell death. Taken together, 
      our results suggest that rpr and hid are expressed together and cooperate to 
      induce programmed cell death during development of the central nervous system 
      midline.
FAU - Zhou, L
AU  - Zhou L
AD  - Biology Department, University of Massachusetts at Amherst, Amherst, MA 01003, 
      USA.
FAU - Schnitzler, A
AU  - Schnitzler A
FAU - Agapite, J
AU  - Agapite J
FAU - Schwartz, L M
AU  - Schwartz LM
FAU - Steller, H
AU  - Steller H
FAU - Nambu, J R
AU  - Nambu JR
LA  - eng
GR  - R01 GM040458/GM/NIGMS NIH HHS/United States
GR  - AG55118/AG/NIA NIH HHS/United States
GR  - GM40458/GM/NIGMS NIH HHS/United States
GR  - NS32251/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Drosophila Proteins)
RN  - 0 (Peptides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (rpr protein, Drosophila)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Crosses, Genetic
MH  - Drosophila/cytology/genetics/*physiology
MH  - *Drosophila Proteins
MH  - Embryo, Nonmammalian/cytology/physiology
MH  - *Gene Expression Regulation, Developmental
MH  - *Genes, Insect/*genetics/physiology
MH  - Multigene Family
MH  - Nervous System/cytology/embryology
MH  - *Nervous System Physiological Phenomena
MH  - Neuroglia/cytology/physiology
MH  - Peptide Biosynthesis
MH  - Peptides/*genetics
MH  - Recombinant Proteins/biosynthesis
MH  - beta-Galactosidase/biosynthesis
PMC - PMC24643
EDAT- 1997/05/13 00:00
MHDA- 1997/05/13 00:01
PMCR- 1997/11/13
CRDT- 1997/05/13 00:00
PHST- 1997/05/13 00:00 [pubmed]
PHST- 1997/05/13 00:01 [medline]
PHST- 1997/05/13 00:00 [entrez]
PHST- 1997/11/13 00:00 [pmc-release]
AID - 0895 [pii]
AID - 10.1073/pnas.94.10.5131 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1997 May 13;94(10):5131-6. doi: 
      10.1073/pnas.94.10.5131.