PMID- 9150365
OWN - NLM
STAT- MEDLINE
DCOM- 19970603
LR  - 20161124
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 14
IP  - 16
DP  - 1997 Apr 24
TI  - Cyclin D1 expression is a major target of the cAMP-induced inhibition of cell 
      cycle entry in fibroblasts.
PG  - 1981-90
AB  - We previously described in the CCL39 hamster fibroblast cell line the inhibition 
      of DNA synthesis reinitiation by agents that elevate cyclic AMP. Here, we show 
      that 8Br-cAMP strongly blocks both the growth factor-induced increase in cyclin 
      D1 protein expression and decrease in p27(KIP1) protein levels, leaving untouched 
      the levels of cyclin D3, cdk2 and cdk4. To assess the role of cyclin D1 in the 
      cAMP-mediated inhibition of DNA synthesis, we overexpressed the cyclin D1 gene in 
      CCL39 and analysed the cAMP response in stable transfectants. We showed that the 
      kinase activities associated to G1 cyclin-cdk complexes are significantly more 
      resistant to cAMP in cyclin D1 transfectants than in their normal counterparts, 
      although the serum-induced p27(KIP1) disparition is still cAMP sensitive in 
      cyclin D1 overexpressors. Interestingly, the mitogen-induced DNA synthesis 
      reinitiation is also much less inhibited by cAMP in cyclin D1 transfectants than 
      in control cells. These data clearly establish that the cAMP-inducible blockade 
      of the G1 phase of the cell cycle can be partially alleviated by overexpression 
      of cyclin D1 in hamster fibroblasts, thus strongly suggesting that cyclin D1 
      protein is one of the major targets for cAMP inhibitory action in fibroblasts.
FAU - L'Allemain, G
AU  - L'Allemain G
AD  - Centre de Biochimie, CNRS-UMR134, Faculte des Sciences, Nice, France.
FAU - Lavoie, J N
AU  - Lavoie JN
FAU - Rivard, N
AU  - Rivard N
FAU - Baldin, V
AU  - Baldin V
FAU - Pouyssegur, J
AU  - Pouyssegur J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 136601-57-5 (Cyclin D1)
RN  - 147604-94-2 (Cyclin-Dependent Kinase Inhibitor p27)
RN  - 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 4)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - IM
MH  - 8-Bromo Cyclic Adenosine Monophosphate/*pharmacology
MH  - Animals
MH  - Cell Cycle/drug effects/*physiology
MH  - *Cell Cycle Proteins
MH  - Cell Line
MH  - Cricetinae
MH  - Cyclic AMP/*metabolism
MH  - Cyclin D1
MH  - Cyclin-Dependent Kinase 4
MH  - Cyclin-Dependent Kinase Inhibitor p27
MH  - Cyclin-Dependent Kinases/antagonists & inhibitors/metabolism
MH  - Cyclins/*biosynthesis/physiology
MH  - DNA Replication/drug effects
MH  - Fibroblasts
MH  - Genes, Tumor Suppressor
MH  - Glutathione Transferase/metabolism
MH  - Kinetics
MH  - Microtubule-Associated Proteins/biosynthesis/metabolism
MH  - Oncogene Proteins/*biosynthesis/physiology
MH  - *Proto-Oncogene Proteins
MH  - Recombinant Proteins/metabolism
MH  - Resting Phase, Cell Cycle
MH  - Retinoblastoma Protein/metabolism
MH  - S Phase
MH  - Transfection
MH  - *Tumor Suppressor Proteins
EDAT- 1997/04/24 00:00
MHDA- 1997/04/24 00:01
CRDT- 1997/04/24 00:00
PHST- 1997/04/24 00:00 [pubmed]
PHST- 1997/04/24 00:01 [medline]
PHST- 1997/04/24 00:00 [entrez]
AID - 10.1038/sj.onc.1201038 [doi]
PST - ppublish
SO  - Oncogene. 1997 Apr 24;14(16):1981-90. doi: 10.1038/sj.onc.1201038.