PMID- 9151854 OWN - NLM STAT- MEDLINE DCOM- 19970609 LR - 20231213 IS - 0022-538X (Print) IS - 1098-5514 (Electronic) IS - 0022-538X (Linking) VI - 71 IP - 6 DP - 1997 Jun TI - The major immediate-early proteins IE1 and IE2 of human cytomegalovirus colocalize with and disrupt PML-associated nuclear bodies at very early times in infected permissive cells. PG - 4599-613 AB - The major immediate-early (MIE) gene products of human cytomegalovirus (HCMV) are nuclear phosphoproteins that are thought to play key roles in initiating lytic cycle gene regulation pathways. We have examined the intranuclear localization pattern of both the IE1 and IE2 proteins in virus-infected and DNA-transfected cells. When HCMV-infected human diploid fibroblast (HF) cells were stained with specific monoclonal antibodies, IE1 localized as a mixture of nuclear diffuse and punctate patterns at very early times (2 h) but changed to an exclusively nuclear diffuse pattern at later times. In contrast, IE2 was distributed predominantly in nuclear punctate structures continuously from 2 to at least 12 h after infection. These punctate structures resembled the preexisting PML-associated nuclear bodies (ND10 or PML oncogenic domains [PODs]) that are disrupted and dispersed by the IE110 protein as a very early event in herpes simplex virus (HSV) infection. However, HCMV differed from HSV by leading instead to a change in both the PML and SP100 protein distribution from punctate bodies to uniform diffuse patterns, a process that was complete in 50% of the cells at 2 h and in 90% of the cells by 4 h after infection. Confocal double-label indirect immunofluorescence assay analysis confirmed that both IE1 and IE2 colocalized transiently with PML in punctate bodies at very early times after infection. In transient expression assays, introduction of IE1-encoding plasmid DNA alone into Vero or HF cells produced the typical total redistribution of PML into a uniform nuclear diffuse pattern together with the IE1 protein, whereas introduction of IE2-encoding plasmid DNA alone resulted in stable colocalization of the IE2 protein with PML in the PODs. A truncated mutant form of IE1 gave large nuclear aggregates and failed to redistribute PML, and similarly a deleted mutant form of IE2 failed to colocalize with the punctate PML bodies, confirming the specificity of these effects. Furthermore, both Vero and U373 cell lines constitutively expressing IE1 also showed total PML relocalization together with the IE1 protein into a nuclear diffuse pattern, although a very small percentage of the cells which failed to express IE1 reverted to a punctate PML pattern. Finally, the PML redistribution activity of IE1 and the direct association of IE2 with PML punctate bodies were both confirmed by infection with E1A-negative recombinant adenovirus vectors expressing either IE1 or IE2 alone. These results confirm that transient colocalization with and disruption of PML-associated nuclear bodies by IE1 and continuous targeting to PML-associated nuclear bodies by IE2 are intrinsic properties of these two MIE regulatory proteins, which we suggest may represent critical initial events for efficient lytic cycle infection by HCMV. FAU - Ahn, J H AU - Ahn JH AD - Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA. FAU - Hayward, G S AU - Hayward GS LA - eng GR - R01 AI24576/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - J Virol JT - Journal of virology JID - 0113724 RN - 0 (Antigens, Nuclear) RN - 0 (Autoantigens) RN - 0 (IE1 protein, cytomegalovirus) RN - 0 (IE2 protein, Cytomegalovirus) RN - 0 (Immediate-Early Proteins) RN - 0 (Membrane Glycoproteins) RN - 0 (Neoplasm Proteins) RN - 0 (Nuclear Proteins) RN - 0 (Promyelocytic Leukemia Protein) RN - 0 (Trans-Activators) RN - 0 (Transcription Factors) RN - 0 (Tumor Suppressor Proteins) RN - 0 (UL115 protein, Human herpesvirus 5) RN - 0 (Viral Envelope Proteins) RN - 0 (Viral Proteins) RN - 0 (glycoprotein H, Cytomegalovirus) RN - 0 (glycoprotein H, Human cytomegalovirus) RN - 0 (glycoprotein O, cytomegalovirus) RN - 135844-47-2 (SP100 protein, human) RN - 143220-95-5 (PML protein, human) SB - IM MH - Animals MH - *Antigens, Nuclear MH - Autoantigens/metabolism MH - Cell Compartmentation MH - Cell Nucleus/*metabolism/ultrastructure MH - Chlorocebus aethiops MH - Cytomegalovirus/*metabolism MH - Fluorescent Antibody Technique, Indirect MH - Humans MH - Immediate-Early Proteins/*metabolism MH - *Membrane Glycoproteins MH - Microscopy, Confocal MH - *Neoplasm Proteins MH - Nuclear Proteins/metabolism MH - Promyelocytic Leukemia Protein MH - Time Factors MH - *Trans-Activators MH - Transcription Factors/*metabolism MH - Tumor Suppressor Proteins MH - Vero Cells MH - *Viral Envelope Proteins MH - *Viral Proteins PMC - PMC191682 EDAT- 1997/06/01 00:00 MHDA- 1997/06/01 00:01 PMCR- 1997/06/01 CRDT- 1997/06/01 00:00 PHST- 1997/06/01 00:00 [pubmed] PHST- 1997/06/01 00:01 [medline] PHST- 1997/06/01 00:00 [entrez] PHST- 1997/06/01 00:00 [pmc-release] AID - 10.1128/JVI.71.6.4599-4613.1997 [doi] PST - ppublish SO - J Virol. 1997 Jun;71(6):4599-613. doi: 10.1128/JVI.71.6.4599-4613.1997.