PMID- 9160753
OWN - NLM
STAT- MEDLINE
DCOM- 19970612
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 89
IP  - 4
DP  - 1997 May 16
TI  - Transcription factor Sp1 is essential for early embryonic development but 
      dispensable for cell growth and differentiation.
PG  - 619-28
AB  - Transcription factor Sp1 has been implicated in the expression of many genes. 
      Moreover, it has been suggested that Sp1 is linked to the maintenance of 
      methylation-free CpG islands, the cell cycle, and the formation of active 
      chromatin structures. We have inactivated the mouse Sp1 gene. Sp1-/- embryos are 
      retarded in development, show a broad range of abnormalities, and die around day 
      11 of gestation. In Sp1-/- embryos, the expression of many putative target genes, 
      including cell cycle-regulated genes, is not affected, CpG islands remain 
      methylation free, and active chromatin is formed at the globin loci. However, the 
      expression of the methyl-CpG-binding protein MeCP2 is greatly reduced in Sp1-/- 
      embryos. MeCP2 is thought to be required for the maintenance of differentiated 
      cells. We suggest that Sp1 is an important regulator of this process.
FAU - Marin, M
AU  - Marin M
AD  - Erasmus University Rotterdam, Department of Cell Biology, The Netherlands.
FAU - Karis, A
AU  - Karis A
FAU - Visser, P
AU  - Visser P
FAU - Grosveld, F
AU  - Grosveld F
FAU - Philipsen, S
AU  - Philipsen S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA Primers)
RN  - 0 (Sp1 Transcription Factor)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Differentiation/genetics/physiology
MH  - Cell Division/genetics/physiology
MH  - Chimera
MH  - CpG Islands
MH  - DNA Methylation
MH  - DNA Primers/genetics
MH  - Embryonic and Fetal Development/genetics/*physiology
MH  - Female
MH  - Gene Targeting
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Pregnancy
MH  - Sp1 Transcription Factor/genetics/*physiology
EDAT- 1997/05/16 00:00
MHDA- 1997/05/16 00:01
CRDT- 1997/05/16 00:00
PHST- 1997/05/16 00:00 [pubmed]
PHST- 1997/05/16 00:01 [medline]
PHST- 1997/05/16 00:00 [entrez]
AID - S0092-8674(00)80243-3 [pii]
AID - 10.1016/s0092-8674(00)80243-3 [doi]
PST - ppublish
SO  - Cell. 1997 May 16;89(4):619-28. doi: 10.1016/s0092-8674(00)80243-3.