PMID- 9168886
OWN - NLM
STAT- MEDLINE
DCOM- 19970619
LR  - 20211203
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 231
IP  - 2
DP  - 1997 May 12
TI  - Inhibition of cellular Cdk2 activity blocks human cytomegalovirus replication.
PG  - 239-47
AB  - Human cytomegalovirus is a herpesvirus that induces numerous cellular processes 
      upon infection. Among these are activation of cyclin-dependent kinase 2, which 
      regulates cell cycle progression in G1 and S phase. We report here that 
      inhibition of cellular Cdk2 activity blocks HCMV replication. Inhibition of Cdk2 
      activity by roscovitine inhibits HCMV DNA synthesis, production of infectious 
      progeny, and late antigen expression in infected cells in a dose-dependent 
      manner. HCMV replication is also inhibited by the expression of a Cdk2 dominant 
      negative mutant, whereas expression of wild-type Cdk2 has no effect on viral 
      replication. These data indicate that activation of cellular Cdk2 is necessary 
      for HCMV replication.
FAU - Bresnahan, W A
AU  - Bresnahan WA
AD  - Department of Microbiology and Immunology, University of Texas Medical Branch, 
      Galveston 77550, USA.
FAU - Boldogh, I
AU  - Boldogh I
FAU - Chi, P
AU  - Chi P
FAU - Thompson, E A
AU  - Thompson EA
FAU - Albrecht, T
AU  - Albrecht T
LA  - eng
GR  - AG10514/AG/NIA NIH HHS/United States
GR  - CA24347/CA/NCI NIH HHS/United States
GR  - DE11389/DE/NIDCR NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antigens, Viral)
RN  - 0 (Cyclins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Purines)
RN  - 0ES1C2KQ94 (Roscovitine)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.22 (CDC2-CDC28 Kinases)
RN  - EC 2.7.11.22 (CDK2 protein, human)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 2)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - IM
MH  - Antigens, Viral/biosynthesis
MH  - *CDC2-CDC28 Kinases
MH  - Cell Line
MH  - Cyclin-Dependent Kinase 2
MH  - Cyclin-Dependent Kinases/*antagonists & inhibitors
MH  - Cyclins/metabolism
MH  - Cytomegalovirus/*growth & development
MH  - Enzyme Activation
MH  - Enzyme Inhibitors/pharmacology
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - Protein Serine-Threonine Kinases/*antagonists & inhibitors
MH  - Purines/pharmacology
MH  - Roscovitine
MH  - Virus Replication/*drug effects
EDAT- 1997/05/12 00:00
MHDA- 1997/05/12 00:01
CRDT- 1997/05/12 00:00
PHST- 1997/05/12 00:00 [pubmed]
PHST- 1997/05/12 00:01 [medline]
PHST- 1997/05/12 00:00 [entrez]
AID - S0042-6822(97)98489-0 [pii]
AID - 10.1006/viro.1997.8489 [doi]
PST - ppublish
SO  - Virology. 1997 May 12;231(2):239-47. doi: 10.1006/viro.1997.8489.