PMID- 9192803
OWN - NLM
STAT- MEDLINE
DCOM- 19970715
LR  - 20061115
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 57
IP  - 12
DP  - 1997 Jun 15
TI  - Human patched (PTCH) mRNA is overexpressed consistently in tumor cells of both 
      familial and sporadic basal cell carcinoma.
PG  - 2336-40
AB  - Recently, a human homologue of the Drosophila patched gene, PTCH, was identified 
      as a putative tumor suppressor mutated in both hereditary and sporadic basal cell 
      carcinomas. Because PTCH controls its own transcription, inactivating mutations 
      in PTCH may lead to overexpression of mutant PTCH mRNA due to loss of 
      autoregulation. The present study is aimed at evaluating whether deregulation of 
      PTCH mRNA expression is a general feature of BCCs of varying histological growth 
      pattern and malignant potential. Irrespective of histological subtype, PTCH mRNA 
      was overexpressed consistently as determined by in situ hybridization in all of 
      the sporadic (n = 16) and hereditary (n = 20) tumors examined. PTCH expression 
      was found in all of the tumor cells but appeared stronger in the peripheral 
      palisading cells. PTCH mRNA was not detected in adjacent nontumor epidermal cells 
      or in other parts of the epidermis. In the majority of tumors (20 of 36), nuclear 
      immunostaining for p53 was found in scattered cells, whereas seven tumors 
      completely lacked p53 immunoreactivity. Our finding of an up-regulation of PTCH 
      mRNA levels in all of the BCCs analyzed indicates that deregulation of the PTCH 
      signaling pathway constitutes an early rate-limiting event in BCC development.
FAU - Unden, A B
AU  - Unden AB
AD  - Department of Dermatology, Karolinska Hospital, Stockholm, Sweden.
FAU - Zaphiropoulos, P G
AU  - Zaphiropoulos PG
FAU - Bruce, K
AU  - Bruce K
FAU - Toftgard, R
AU  - Toftgard R
FAU - Stahle-Backdahl, M
AU  - Stahle-Backdahl M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (ptc protein, Drosophila)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Basal Cell/genetics/*metabolism
MH  - *Drosophila Proteins
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Insect Proteins/*metabolism
MH  - Male
MH  - Membrane Proteins/*metabolism
MH  - Middle Aged
MH  - RNA, Messenger/metabolism
MH  - Receptors, Cell Surface
MH  - Skin Neoplasms/genetics/*metabolism
MH  - Tumor Suppressor Protein p53/metabolism
EDAT- 1997/06/15 00:00
MHDA- 1997/06/15 00:01
CRDT- 1997/06/15 00:00
PHST- 1997/06/15 00:00 [pubmed]
PHST- 1997/06/15 00:01 [medline]
PHST- 1997/06/15 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1997 Jun 15;57(12):2336-40.