PMID- 9215627
OWN - NLM
STAT- MEDLINE
DCOM- 19970807
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 89
IP  - 7
DP  - 1997 Jun 27
TI  - Proteolysis that is inhibited by hedgehog targets Cubitus interruptus protein to 
      the nucleus and converts it to a repressor.
PG  - 1043-53
AB  - Cell-cell communication at anterior/posterior compartment borders in Drosophila 
      involves Hedgehog (Hh), a protein secreted by posterior cells, and Cubitus 
      interruptus (Ci), a protein in the Hh response pathway in anterior cells. 
      Although Ci is thought to have roles as a transcription factor repressing hh 
      expression and activating target genes, it localizes in the cytoplasm of anterior 
      cells. We report here the identification of a domain that tethers Ci in the 
      cytoplasm and show that in some anterior cells, Ci is cleaved to generate a form 
      that lacks the tethering domain. This form translocates to the nucleus where it 
      represses hh and other target genes. Hh inhibits proteolysis of Ci, and we 
      suggest that this inhibition leads to the observed patterns of expression of key 
      target genes at the compartment border.
FAU - Aza-Blanc, P
AU  - Aza-Blanc P
AD  - Department of Biochemistry and Biophysics, University of California, San 
      Francisco 94143, USA.
FAU - Ramirez-Weber, F A
AU  - Ramirez-Weber FA
FAU - Laget, M P
AU  - Laget MP
FAU - Schwartz, C
AU  - Schwartz C
FAU - Kornberg, T B
AU  - Kornberg TB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (ci protein, Drosophila)
RN  - 0 (ptc protein, Drosophila)
RN  - 149291-21-4 (hh protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/chemistry/metabolism
MH  - Cells, Cultured
MH  - Cytoplasm/chemistry/metabolism
MH  - DNA-Binding Proteins/chemistry/*metabolism
MH  - Drosophila/chemistry/embryology/*physiology
MH  - *Drosophila Proteins
MH  - Embryo, Nonmammalian/chemistry/physiology
MH  - Gene Expression Regulation, Developmental/physiology
MH  - Hedgehog Proteins
MH  - Insect Proteins/genetics/*metabolism
MH  - Membrane Proteins/metabolism
MH  - Protein Structure, Tertiary
MH  - Receptors, Cell Surface/metabolism
MH  - Repressor Proteins/*metabolism
MH  - Signal Transduction/physiology
MH  - Transcription Factors
MH  - Zinc Fingers/physiology
EDAT- 1997/06/27 00:00
MHDA- 1997/06/27 00:01
CRDT- 1997/06/27 00:00
PHST- 1997/06/27 00:00 [pubmed]
PHST- 1997/06/27 00:01 [medline]
PHST- 1997/06/27 00:00 [entrez]
AID - S0092-8674(00)80292-5 [pii]
AID - 10.1016/s0092-8674(00)80292-5 [doi]
PST - ppublish
SO  - Cell. 1997 Jun 27;89(7):1043-53. doi: 10.1016/s0092-8674(00)80292-5.