PMID- 9245788
OWN - NLM
STAT- MEDLINE
DCOM- 19970828
LR  - 20240214
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 138
IP  - 3
DP  - 1997 Aug 11
TI  - Drosophila myoblast city encodes a conserved protein that is essential for 
      myoblast fusion, dorsal closure, and cytoskeletal organization.
PG  - 589-603
AB  - The Drosophila myoblast city (mbc) locus was previously identified on the basis 
      of a defect in myoblast fusion (Rushton et al., 1995. Development [Camb.]. 
      121:1979-1988). We describe herein the isolation and characterization of the mbc 
      gene. The mbc transcript and its encoded protein are expressed in a broad range 
      of tissues, including somatic myoblasts, cardial cells, and visceral mesoderm. It 
      is also expressed in the pole cells and in ectodermally derived tissues, 
      including the epidermis. Consistent with this latter expression, mbc mutant 
      embryos exhibit defects in dorsal closure and cytoskeletal organization in the 
      migrating epidermis. Both the mesodermal and ectodermal defects are reminiscent 
      of those induced by altered forms of Drac1 and suggest that mbc may function in 
      the same pathway. MBC bears striking homology to human DOCK180, which interacts 
      with the SH2-SH3 adapter protein Crk and may play a role in signal transduction 
      from focal adhesions. Taken together, these results suggest the possibility that 
      MBC is an intermediate in a signal transduction pathway from the rho/rac family 
      of GTPases to events in the cytoskeleton and that this pathway may be used during 
      myoblast fusion and dorsal closure.
FAU - Erickson, M R
AU  - Erickson MR
AD  - Department of Biochemistry and Molecular Biology and Center for Gene Regulation, 
      The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
FAU - Galletta, B J
AU  - Galletta BJ
FAU - Abmayr, S M
AU  - Abmayr SM
LA  - eng
SI  - GENBANK/AA110899
SI  - GENBANK/AF007805
SI  - GENBANK/D86964
SI  - GENBANK/Z81032
SI  - GENBANK/Z81054
GR  - R01 AR044274/AR/NIAMS NIH HHS/United States
GR  - R01 AR44274/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DOCK1 protein, human)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Proteins)
RN  - 0 (mbc protein, Drosophila)
RN  - EC 3.6.5.2 (rac GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Fusion
MH  - Cell Movement
MH  - *Cytoskeletal Proteins
MH  - Cytoskeleton/*ultrastructure
MH  - Drosophila/chemistry/embryology/*genetics
MH  - *Drosophila Proteins
MH  - Ectoderm/metabolism
MH  - Embryo, Nonmammalian/*metabolism
MH  - Embryonic Development
MH  - Epidermis/embryology/metabolism
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Genes, Insect
MH  - Humans
MH  - Insect Proteins/chemistry/*genetics/*physiology
MH  - Male
MH  - Mesoderm/metabolism
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Muscle Fibers, Skeletal/*cytology/metabolism
MH  - Proteins/chemistry
MH  - Sequence Alignment
MH  - Signal Transduction
MH  - *rac GTP-Binding Proteins
PMC - PMC2141626
EDAT- 1997/08/11 00:00
MHDA- 1997/08/11 00:01
PMCR- 1998/02/11
CRDT- 1997/08/11 00:00
PHST- 1997/08/11 00:00 [pubmed]
PHST- 1997/08/11 00:01 [medline]
PHST- 1997/08/11 00:00 [entrez]
PHST- 1998/02/11 00:00 [pmc-release]
AID - 10.1083/jcb.138.3.589 [doi]
PST - ppublish
SO  - J Cell Biol. 1997 Aug 11;138(3):589-603. doi: 10.1083/jcb.138.3.589.