PMID- 9293448
OWN - NLM
STAT- MEDLINE
DCOM- 19971022
LR  - 20071115
IS  - 0300-8630 (Print)
IS  - 0300-8630 (Linking)
VI  - 209
IP  - 4
DP  - 1997 Jul-Aug
TI  - Good engraftment of B-cell precursor ALL in NOD-SCID mice.
PG  - 178-85
AB  - BACKGROUND: In vivo models for human B cell precursor ALL have been established 
      by transplanting human leukemic cells onto immune-deficient SCID mice. High risk 
      and relapsed leukemias engraft very well in these mice, however, good prognosis 
      pediatric ALL often grow poorly if at all. Recently a new, even more 
      immune-deficient mouse strain has been bred by crossing the scid mutation onto 
      the NOD mouse background. As these NOD-SCID mice have been shown to be better 
      recipients for human myeloid cells the goal of this study was to test these mice 
      as hosts for human acute lymphoblastic leukemia cells. PATIENTS AND METHODS: Bone 
      marrow or peripheral blood cells from/pediatric and one adult patient with B-cell 
      precursor ALL were transplanted onto immune-deficient NOD-SCID mice according to 
      established protocols. MAIN RESULTS: ALL cells from 6 out of the 8 patients (75%) 
      successfully engrafted the NOD-SCID mice and from 4 patients (50%) led to an 
      extensive leukemic infiltration in the murine marrow (> 10%). High level human 
      cell engraftment could be demonstrated by flow cytometry, Southern blot analysis 
      and cytology. By cytology and immunophenotype the leukemia in the mice was 
      indistinguishable from the original leukemia in the patients. The presence of few 
      human eosinophils in the marrow of highly engrafted mice indicates minimal 
      coengraftment of residual normal cells. Development of overt leukemia in the mice 
      after transplantation of cells from different patients varied between 1.5 and 7 
      months. Interestingly and in contrast to myeloid cells, conditioning of the mice 
      by sublethal irradiation was not necessary for successful engraftment. Limiting 
      dilution experiments with leukemic blasts from one patient showed that as few as 
      10000 cells were sufficient to transfer the leukemia onto NOD-SCID mice. 
      CONCLUSIONS: NOD-SCID mice are sensitive recipients for human ALL xenografts.
FAU - Baersch, G
AU  - Baersch G
AD  - Klinik und Poliklinik fur Kinderheilkunde, Westfalische Wilhelms-Universitat 
      Munster.
FAU - Mollers, T
AU  - Mollers T
FAU - Hotte, A
AU  - Hotte A
FAU - Dockhorn-Dworniczak, B
AU  - Dockhorn-Dworniczak B
FAU - Rube, C
AU  - Rube C
FAU - Ritter, J
AU  - Ritter J
FAU - Jurgens, H
AU  - Jurgens H
FAU - Vormoor, J
AU  - Vormoor J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Klin Padiatr
JT  - Klinische Padiatrie
JID - 0326144
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Bone Marrow/pathology
MH  - Bone Marrow Transplantation/pathology
MH  - Child
MH  - DNA, Neoplasm/genetics
MH  - Flow Cytometry
MH  - Gene Expression Regulation, Leukemic/physiology
MH  - *Graft Enhancement, Immunologic
MH  - Graft Survival/*physiology
MH  - Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Immunophenotyping
MH  - Leukemic Infiltration/pathology
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/genetics/*pathology
MH  - Transplantation, Heterologous
EDAT- 1997/07/01 00:00
MHDA- 1997/09/18 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/09/18 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1055/s-2008-1043947 [doi]
PST - ppublish
SO  - Klin Padiatr. 1997 Jul-Aug;209(4):178-85. doi: 10.1055/s-2008-1043947.