PMID- 9314525
OWN - NLM
STAT- MEDLINE
DCOM- 19980218
LR  - 20190603
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 139
IP  - 1
DP  - 1997 Oct 6
TI  - Chromosome association of minichromosome maintenance proteins in Drosophila 
      mitotic cycles.
PG  - 13-21
AB  - Minichromosome maintenance (MCM) proteins are essential DNA replication factors 
      conserved among eukaryotes. MCMs cycle between chromatin bound and dissociated 
      states during each cell cycle. Their absence on chromatin is thought to 
      contribute to the inability of a G2 nucleus to replicate DNA. Passage through 
      mitosis restores the ability of MCMs to bind chromatin and the ability to 
      replicate DNA. In Drosophila early embryonic cell cycles, which lack a G1 phase, 
      MCMs reassociate with condensed chromosomes toward the end of mitosis. To explore 
      the coupling between mitosis and MCM-chromatin interaction, we tested whether 
      this reassociation requires mitotic degradation of cyclins. Arrest of mitosis by 
      induced expression of nondegradable forms of cyclins A and/or B showed that 
      reassociation of MCMs to chromatin requires cyclin A destruction but not cyclin B 
      destruction. In contrast to the earlier mitoses, mitosis 16 (M16) is followed by 
      G1, and MCMs do not reassociate with chromatin at the end of M16. dacapo mutant 
      embryos lack an inhibitor of cyclin E, do not enter G1 quiescence after M16, and 
      show mitotic reassociation of MCM proteins. We propose that cyclin E, inhibited 
      by Dacapo in M16, promotes chromosome binding of MCMs. We suggest that cyclins 
      have both positive and negative roles in controlling MCM-chromatin association.
FAU - Su, T T
AU  - Su TT
AD  - Department of Biochemistry and Biophysics, University of California San Francisco 
      94143-0448, USA.
FAU - O'Farrell, P H
AU  - O'Farrell PH
LA  - eng
GR  - R01 GM037193/GM/NIGMS NIH HHS/United States
GR  - 2-RO1-GM37193/GM/NIGMS NIH HHS/United States
GR  - GM15032/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (dap protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Cell Cycle Proteins/*genetics/metabolism/physiology
MH  - Chromosomes/genetics/metabolism/*physiology
MH  - Cyclins/metabolism
MH  - Drosophila
MH  - *Drosophila Proteins
MH  - Insect Proteins/genetics/metabolism
MH  - Mitosis/*genetics/physiology
MH  - Nuclear Proteins/*genetics/metabolism/physiology
PMC - PMC2139827
EDAT- 1997/10/06 00:00
MHDA- 1997/10/06 00:01
PMCR- 1998/04/06
CRDT- 1997/10/06 00:00
PHST- 1997/10/06 00:00 [pubmed]
PHST- 1997/10/06 00:01 [medline]
PHST- 1997/10/06 00:00 [entrez]
PHST- 1998/04/06 00:00 [pmc-release]
AID - 10.1083/jcb.139.1.13 [doi]
PST - ppublish
SO  - J Cell Biol. 1997 Oct 6;139(1):13-21. doi: 10.1083/jcb.139.1.13.