PMID- 9334317
OWN - NLM
STAT- MEDLINE
DCOM- 19971106
LR  - 20240214
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 11
IP  - 19
DP  - 1997 Oct 1
TI  - Coupling of cell division to cell growth by translational control of the G1 
      cyclin CLN3 in yeast.
PG  - 2522-31
AB  - The eukaryotic cell cycle is driven by a cascade of cyclins and kinase partners 
      including the G1 cyclin Cln3p in yeast. As the first step in this cascade, Cln3p 
      is uniquely positioned to determine the critical growth-rate threshold for 
      division. To analyze factors regulating CLN3 expression, we identified a short 
      upstream open reading frame (uORF) in the 5' leader of CLN3 mRNA as a 
      translational control element. This control element is critical for the 
      growth-dependent regulation of Cln3p synthesis because it specifically represses 
      CLN3 expression during conditions of diminished protein synthesis or slow growth. 
      Inactivation of the uORF accelerates the completion of Start and entry into the 
      cell cycle suggesting that translational regulation of CLN3 provides a mechanism 
      coupling cell growth and division.
FAU - Polymenis, M
AU  - Polymenis M
AD  - MGH Cancer Center, Massachusetts General Hospital, Charlestown 02129, USA.
FAU - Schmidt, E V
AU  - Schmidt EV
LA  - eng
GR  - R01 CA063117/CA/NCI NIH HHS/United States
GR  - R01-CA63117/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Antifungal Agents)
RN  - 0 (CLN3 protein, S cerevisiae)
RN  - 0 (Cyclins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Peptide Initiation Factors)
RN  - 0 (Polyenes)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Messenger)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Antifungal Agents/pharmacology
MH  - Cell Division/genetics/*physiology
MH  - Cyclins/biosynthesis/*genetics
MH  - DNA Replication/genetics
MH  - Flow Cytometry
MH  - Fungal Proteins/biosynthesis/*genetics
MH  - *Gene Expression Regulation, Fungal
MH  - Genes, Reporter
MH  - Mutation
MH  - Open Reading Frames/genetics
MH  - Peptide Initiation Factors/genetics
MH  - Polyenes/pharmacology
MH  - Polyribosomes/genetics
MH  - Protein Biosynthesis/genetics
MH  - RNA, Fungal/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Saccharomyces cerevisiae/*genetics/growth & development
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Analysis, DNA
MH  - Sirolimus
PMC - PMC316559
EDAT- 1997/10/23 00:00
MHDA- 1997/10/23 00:01
PMCR- 1998/04/01
CRDT- 1997/10/23 00:00
PHST- 1997/10/23 00:00 [pubmed]
PHST- 1997/10/23 00:01 [medline]
PHST- 1997/10/23 00:00 [entrez]
PHST- 1998/04/01 00:00 [pmc-release]
AID - 10.1101/gad.11.19.2522 [doi]
PST - ppublish
SO  - Genes Dev. 1997 Oct 1;11(19):2522-31. doi: 10.1101/gad.11.19.2522.