PMID- 9356475
OWN - NLM
STAT- MEDLINE
DCOM- 19971216
LR  - 20220311
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 94
IP  - 23
DP  - 1997 Nov 11
TI  - Growth suppression of glioma cells by PTEN requires a functional phosphatase 
      catalytic domain.
PG  - 12479-84
AB  - Deletions of all or part of chromosome 10 are the most common genetic alterations 
      in high-grade gliomas. The PTEN gene (also called MMAC1 and TEP1) maps to 
      chromosome region 10q23 and has been implicated as a target of alteration in 
      gliomas and also in other cancers such as those of the breast, prostate, and 
      kidney. Here we sought to provide a functional test of its candidacy as a growth 
      suppressor in glioma cells. We used a combination of Northern blot analysis, 
      protein truncation assays, and sequence analysis to determine the types and 
      frequency of PTEN mutations in glioma cell lines so that we could define 
      appropriate recipients to assess the growth suppressive function of PTEN by gene 
      transfer. Introduction of wild-type PTEN into glioma cells containing endogenous 
      mutant alleles caused growth suppression, but was without effect in cells 
      containing endogenous wild-type PTEN. The ectopic expression of PTEN alleles, 
      which carried mutations found in primary tumors and have been shown or are 
      expected to inactivate its phosphatase activity, caused little growth 
      suppression. These data strongly suggest that PTEN is a protein phosphatase that 
      exhibits functional and specific growth-suppressing activity.
FAU - Furnari, F B
AU  - Furnari FB
AD  - Ludwig Institute for Cancer Research, University of California at San Diego, La 
      Jolla, CA 92093-0660, USA. ffurnari@ucsd.edu
FAU - Lin, H
AU  - Lin H
FAU - Huang, H S
AU  - Huang HS
FAU - Cavenee, W K
AU  - Cavenee WK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - Brain Neoplasms/genetics/*pathology
MH  - Cell Division/genetics
MH  - *Chromosomes, Human, Pair 10
MH  - *Gene Expression Regulation, Neoplastic
MH  - Glioma/*genetics/*pathology
MH  - Humans
MH  - Mutation
MH  - PTEN Phosphohydrolase
MH  - *Phosphoric Monoester Hydrolases
MH  - Protein Tyrosine Phosphatases/*genetics
MH  - Tumor Cells, Cultured
MH  - *Tumor Suppressor Proteins
PMC - PMC25009
EDAT- 1997/11/14 00:00
MHDA- 1997/11/14 00:01
PMCR- 1998/05/11
CRDT- 1997/11/14 00:00
PHST- 1997/11/14 00:00 [pubmed]
PHST- 1997/11/14 00:01 [medline]
PHST- 1997/11/14 00:00 [entrez]
PHST- 1998/05/11 00:00 [pmc-release]
AID - 3016 [pii]
AID - 10.1073/pnas.94.23.12479 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12479-84. doi: 
      10.1073/pnas.94.23.12479.