PMID- 9359477
OWN - NLM
STAT- MEDLINE
DCOM- 19971117
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 73
IP  - 3
DP  - 1997 Nov 4
TI  - Increased hyaluronidase levels in breast tumor metastases.
PG  - 327-31
AB  - Hyaluronidase, a matrix-degrading enzyme, was assayed in extracts from breast 
      primary tumors and regional metastases using a pool of human sera as a standard. 
      Optimal activities of tumor extracts and serum were found for concentrations of 
      0.15-0.20 M NaCl in pH 3.8-4.0 buffer. In evaluating contamination by serum due 
      to vascular proliferation, we expressed our results as the ratio of the entire 
      activity (mU/l extract) on serum albumin content of tumors (g/l). Median 
      (interquartile range) activities were 9.02 (6.04-14.34) for primary tumors and 
      37.36 (24.06-99.63) mU/g albumin for metastases. The difference was significant. 
      Zymographic analysis showed that 3 bands of activity were detected which 
      corresponded to 68, 53 and 49 kDa for tumoral hyaluronidase. The same pattern was 
      observed for cellular extracts of breast cancer cell line CAL51, demonstrating 
      that hyaluronidase detected in tumor extracts had mainly a cellular origin. Our 
      results suggest that hyaluronidase may be involved in the metastatic process.
FAU - Bertrand, P
AU  - Bertrand P
AD  - Department of Molecular Oncology, Centre Henri-Becquerel, Rouen, France.
FAU - Girard, N
AU  - Girard N
FAU - Duval, C
AU  - Duval C
FAU - d'Anjou, J
AU  - d'Anjou J
FAU - Chauzy, C
AU  - Chauzy C
FAU - Menard, J F
AU  - Menard JF
FAU - Delpech, B
AU  - Delpech B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Neoplasm Proteins)
RN  - EC 3.2.1.35 (Hyaluronoglucosaminidase)
SB  - IM
MH  - Breast Neoplasms/blood/*enzymology/pathology
MH  - Female
MH  - Humans
MH  - Hyaluronoglucosaminidase/*analysis/blood
MH  - Lymphatic Metastasis
MH  - Neoplasm Proteins/*analysis/blood
MH  - Tumor Cells, Cultured/enzymology
EDAT- 1997/11/14 00:28
MHDA- 2000/06/20 09:00
CRDT- 1997/11/14 00:28
PHST- 1997/11/14 00:28 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/11/14 00:28 [entrez]
AID - 10.1002/(SICI)1097-0215(19971104)73:3<327::AID-IJC4>3.0.CO;2-1 [pii]
AID - 10.1002/(sici)1097-0215(19971104)73:3<327::aid-ijc4>3.0.co;2-1 [doi]
PST - ppublish
SO  - Int J Cancer. 1997 Nov 4;73(3):327-31. doi: 
      10.1002/(sici)1097-0215(19971104)73:3<327::aid-ijc4>3.0.co;2-1.