PMID- 9370456
OWN - NLM
STAT- MEDLINE
DCOM- 19980116
LR  - 20220317
IS  - 0006-3495 (Print)
IS  - 1542-0086 (Electronic)
IS  - 0006-3495 (Linking)
VI  - 73
IP  - 5
DP  - 1997 Nov
TI  - Exchange of monooleoylphosphatidylcholine as monomer and micelle with membranes 
      containing poly(ethylene glycol)-lipid.
PG  - 2615-29
AB  - Surface-grafted polymers, such as poly(ethylene glycol) (PEG), provide an 
      effective steric barrier against surface-surface and surface-macromolecule 
      interactions. In the present work, we have studied the exchange of 
      monooleoylphosphatidylcholine (MOPC) with vesicle membranes containing 750 mol wt 
      surface-grafted PEG (incorporated as PEG-lipid) from 0 to 20 mol % and have 
      analyzed the experimental results in terms of thermodynamic and stationary 
      equilibrium models. Micropipette manipulation was used to expose a single lipid 
      vesicle to a flow of MOPC solution (0.025 microM to 500 microM). MOPC uptake was 
      measured by a direct measure of the vesicle area change. The presence of PEG(750) 
      lipid in the vesicle membrane inhibited the partitioning of MOPC micelles (and to 
      some extent microaggregates) into the membrane, while even up to 20 mol % 
      PEG-lipid, it did not affect the exchange of MOPC monomers both into and out of 
      the membrane. The experimental data and theoretical models show that grafted PEG 
      acts as a very effective molecular scale "filter" and prevents micelle-membrane 
      contact, substantially decreasing the apparent rate and amount of MOPC taken up 
      by the membrane, thereby stabilizing the membrane in a solution of MOPC that 
      would otherwise dissolve it.
FAU - Needham, D
AU  - Needham D
AD  - Department of Mechanical Engineering and Materials Science, Duke University, 
      Durham, North Carolina 27708-0300, USA.
FAU - Stoicheva, N
AU  - Stoicheva N
FAU - Zhelev, D V
AU  - Zhelev DV
LA  - eng
GR  - GM 40162/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biophys J
JT  - Biophysical journal
JID - 0370626
RN  - 0 (Lipid Bilayers)
RN  - 0 (Liposomes)
RN  - 0 (Lysophosphatidylcholines)
RN  - 0 (Micelles)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Phosphatidylethanolamines)
RN  - 0 (Phospholipids)
RN  - 133906-05-5 (dioleoyl-N-(monomethoxypolyethylene glycol 
      succinyl)phosphatidylethanolamine)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 6753-56-6 (1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine)
SB  - IM
MH  - Kinetics
MH  - Lipid Bilayers/*chemistry/metabolism
MH  - Liposomes/chemistry/metabolism
MH  - Lysophosphatidylcholines/*chemistry/metabolism
MH  - Membrane Fusion
MH  - Micelles
MH  - Microscopy, Video
MH  - Models, Biological
MH  - Phosphatidylcholines/metabolism
MH  - Phosphatidylethanolamines/*chemistry/metabolism
MH  - Phospholipids/chemistry/metabolism
MH  - Polyethylene Glycols/*chemistry/metabolism
MH  - Surface Properties
MH  - Thermodynamics
PMC - PMC1181164
EDAT- 1997/11/25 00:00
MHDA- 1997/11/25 00:01
PMCR- 1998/11/01
CRDT- 1997/11/25 00:00
PHST- 1997/11/25 00:00 [pubmed]
PHST- 1997/11/25 00:01 [medline]
PHST- 1997/11/25 00:00 [entrez]
PHST- 1998/11/01 00:00 [pmc-release]
AID - S0006-3495(97)78291-2 [pii]
AID - 10.1016/S0006-3495(97)78291-2 [doi]
PST - ppublish
SO  - Biophys J. 1997 Nov;73(5):2615-29. doi: 10.1016/S0006-3495(97)78291-2.