PMID- 9413151
OWN - NLM
STAT- MEDLINE
DCOM- 19980120
LR  - 20220223
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 17
IP  - 5A
DP  - 1997 Sep-Oct
TI  - Interleukin 10 is expressed in human gliomas in vivo and increases glioma cell 
      proliferation and motility in vitro.
PG  - 3217-24
AB  - Interleukin 10 (IL-10) is a cytokine with a broad spectrum of immunosuppressive 
      activity, but itoffs role in the oncogenesis of solid tumors is still unclear. In 
      previous experiments we have shown that IL-10 specific mRNA is produced within 
      glial tumors in vivo. The aim of the present study was to investigate the 
      expression of the IL-10 protein in vivo and to identify the cells producing IL-10 
      within the tumor tissue. Expression levels significantly increased with 
      malignancy of the gliomas. 87.5% of grade III and IV, but only 4% of grade II 
      tumors expressed high levels of mRNA. Elevation of IL-10 serum levels was found 
      in 11% of low grade and in 63.6% of high grade glioma patients. In situ 
      hybridization analysis with combined immunohistochemistry revealed that: a) IL-10 
      is not produced by infiltrating B- or T- lymphocytes, b) both microglia and 
      astroglia contributed to IL-10 expression in malignant gliomas in vivo. These 
      data suggested the functional role of IL-10 in glioma progression. Therefore, the 
      effects of IL-10 on proliferation and migration of glioma cells were determined 
      in vitro. Two human glioma cell lines were grown as monolayer as well as 
      spheroids in the presence of different concentrations of IL-10. IL-10 increased 
      cell proliferation significantly in both culture systems with a dose optimum of 
      25 ng/ml. Glioma cell motility was enhanced with 25 ng/ml as the optimal dose. 
      Adding the IL-10 specific antibody reversed both effects. We conclude from our 
      data that IL-10 is involved in the progression of glial tumors, especially in the 
      enhancement of tumor cell proliferation and migration which promotes infiltration 
      of the surrounding tissue.
FAU - Huettner, C
AU  - Huettner C
AD  - Institute of Pathology, University of Wuerzburg, Germany.
FAU - Czub, S
AU  - Czub S
FAU - Kerkau, S
AU  - Kerkau S
FAU - Roggendorf, W
AU  - Roggendorf W
FAU - Tonn, J C
AU  - Tonn JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Brain/embryology
MH  - Cell Division
MH  - Cell Movement
MH  - Glioma/*metabolism/pathology
MH  - Humans
MH  - In Situ Hybridization
MH  - Interleukin-10/*metabolism
MH  - Organoids
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Messenger/metabolism
MH  - RNA, Neoplasm/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1997/12/31 00:00
MHDA- 1997/12/31 00:01
CRDT- 1997/12/31 00:00
PHST- 1997/12/31 00:00 [pubmed]
PHST- 1997/12/31 00:01 [medline]
PHST- 1997/12/31 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1997 Sep-Oct;17(5A):3217-24.