PMID- 9435286
OWN - NLM
STAT- MEDLINE
DCOM- 19980402
LR  - 20220215
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 125
IP  - 4
DP  - 1998 Feb
TI  - zfh-1 is required for germ cell migration and gonadal mesoderm development in 
      Drosophila.
PG  - 655-66
AB  - In Drosophila as well as many vertebrate systems, germ cells form 
      extraembryonically and migrate into the embryo before navigating toward gonadal 
      mesodermal cells. How the gonadal mesoderm attracts migratory germ cells is not 
      understood in any system. We have taken a genetic approach to identify genes 
      required for germ cell migration in Drosophila. Here we describe the role of 
      zfh-1 in germ cell migration to the gonadal mesoderm. In zfh-1 mutant embryos, 
      the initial association of germ cells and gonadal mesoderm is blocked. Loss of 
      zfh-1 activity disrupts the development of two distinct mesodermal populations: 
      the caudal visceral mesoderm and the gonadal mesoderm. We demonstrate that the 
      caudal visceral mesoderm facilitates the migration of germ cells from the 
      endoderm to the mesoderm. Zfh-1 is also expressed in the gonadal mesoderm 
      throughout the development of this tissue. Ectopic expression of Zfh-1 is 
      sufficient to induce additional gonadal mesodermal cells and to alter the 
      temporal course of gene expression within these cells. Finally, through analysis 
      of a tinman zfh-1 double mutant, we show that zfh-1 acts in conjunction with 
      tinman, another homeodomain protein, in the specification of lateral mesodermal 
      derivatives, including the gonadal mesoderm.
FAU - Broihier, H T
AU  - Broihier HT
AD  - Skirball Institute, New York University Medical Center, NY, NY 10016, USA.
FAU - Moore, L A
AU  - Moore LA
FAU - Van Doren, M
AU  - Van Doren M
FAU - Newman, S
AU  - Newman S
FAU - Lehmann, R
AU  - Lehmann R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (tin protein, Drosophila)
RN  - 0 (zfh1 protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Cell Movement/genetics/physiology
MH  - DNA-Binding Proteins/*genetics/*physiology
MH  - Drosophila/cytology/*embryology/*genetics
MH  - *Drosophila Proteins
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Genes, Insect
MH  - Germ Cells/*physiology
MH  - Gonads/*cytology/*embryology
MH  - Homeodomain Proteins/genetics/physiology
MH  - In Situ Hybridization
MH  - Male
MH  - Mesoderm/cytology
MH  - Mutation
MH  - *Repressor Proteins
MH  - *Trans-Activators
EDAT- 1998/04/04 00:00
MHDA- 1998/04/04 00:01
CRDT- 1998/04/04 00:00
PHST- 1998/04/04 00:00 [pubmed]
PHST- 1998/04/04 00:01 [medline]
PHST- 1998/04/04 00:00 [entrez]
AID - 10.1242/dev.125.4.655 [doi]
PST - ppublish
SO  - Development. 1998 Feb;125(4):655-66. doi: 10.1242/dev.125.4.655.