PMID- 9439980
OWN - NLM
STAT- MEDLINE
DCOM- 19980217
LR  - 20220317
IS  - 1082-8893 (Print)
IS  - 1082-8893 (Linking)
VI  - 11
IP  - 1
DP  - 1997-1998
TI  - Curcumin inhibits IL1 alpha and TNF-alpha induction of AP-1 and NF-kB DNA-binding 
      activity in bone marrow stromal cells.
PG  - 49-62
AB  - We have previously demonstrated that anti-inflammatory and antioxidant compound 
      curcumin (diferuloyl-methane) inhibits the expression of monocyte chemoattractant 
      protein-1 (MCP-1/JE) in bone marrow stromal cells by suppressing the 
      transcriptional activity of the MCP-1/JE gene. Since both AP-1 (TRE) and NF-kB 
      (kB) binding motifs are present in the promoter of MCP-1/JE gene, we examined the 
      effect of curcumin on IL1 alpha- and TNF-alpha-induced activation of ubiquitous 
      transcription factors AP-1 and NF-kB by electrophoretic mobility shift assay and 
      Western blotting. IL1 alpha and TNF-alpha rapidly induced both AP-1 and NF-kB DNA 
      binding activities in +/+(-)1.LDA11 stromal cells. However, treatment of these 
      cells with curcumin blocked the activation of AP-1 and NF-kB by both cytokines. 
      These data suggest that inhibition of MCP-1/JE transcription by curcumin involves 
      blocking of AP-1 and NF-kB activation by IL1 alpha or TNF-alpha.
FAU - Xu, Y X
AU  - Xu YX
AD  - Division of Hematology/Oncology, Henry Ford Hospital, Detroit, MI 48202, USA.
FAU - Pindolia, K R
AU  - Pindolia KR
FAU - Janakiraman, N
AU  - Janakiraman N
FAU - Chapman, R A
AU  - Chapman RA
FAU - Gautam, S C
AU  - Gautam SC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hematopathol Mol Hematol
JT  - Hematopathology and molecular hematology
JID - 9608785
RN  - 0 (Antineoplastic Agents)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Interleukin-1)
RN  - 0 (NF-kappa B)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*pharmacology
MH  - Bone Marrow Cells/*metabolism
MH  - Cells, Cultured
MH  - Curcumin/*pharmacology
MH  - DNA-Binding Proteins/metabolism
MH  - Drug Interactions
MH  - Interleukin-1/*pharmacology
MH  - Mice
MH  - NF-kappa B/*metabolism
MH  - Stromal Cells/*metabolism
MH  - Transcription Factor AP-1/*metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1997/01/01 00:00
MHDA- 1998/01/24 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1998/01/24 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
PST - ppublish
SO  - Hematopathol Mol Hematol. 1997-1998;11(1):49-62.