PMID- 9461298
OWN - NLM
STAT- MEDLINE
DCOM- 19980227
LR  - 20220317
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 250
IP  - 3
DP  - 1997 Dec 15
TI  - Membrane-type matrix metalloproteinases 1 and 2 exhibit broad-spectrum 
      proteolytic capacities comparable to many matrix metalloproteinases.
PG  - 751-7
AB  - Soluble proenzyme forms of the catalytic domains of membrane-type matrix 
      metalloproteinases 1 and 2 (MT1-MMP and MT2-MMP) and a form of MT1-MMP containing 
      the catalytic and hemopexin domains were expressed as soluble recombinant 
      proteins. Purified, activated forms of the MT-MMP were shown to degrade 
      fibronectin, tenascin, nidogen, aggrecan and perlecan. Only MT2-MMP showed 
      activity against laminin. MT1-MMP retaining the hemopexin domain was able to 
      specifically cleave native type-I and type-III collagens into the 3/4-1/4 
      fragments typical of the specific collagenases. The catalytic domain alone did 
      not retain this activity. The MT-MMP did not degrade interleukin-1beta, but, 
      similarly to many other MMP, could process a pro [tumor necrosis factor (TNF) 
      alpha] fusion protein to release mature TNF. However, the latter was subsequently 
      degraded into smaller fragments. These results demonstrate that, in addition to 
      their ability to activate other MMP, such as progelatinase A/proMMP2 and 
      procollagenase-3/proMMP13, MT-MMP degrade a number of extracellular matrix 
      macromolecules. Their location at the surface of cells implies that they could 
      play a significant role in the modulation of cell-matrix interactions.
FAU - d'Ortho, M P
AU  - d'Ortho MP
AD  - INSERM U296, Faculte de Medecine, Creteil, France. dortho@im3.inserm.fr
FAU - Will, H
AU  - Will H
FAU - Atkinson, S
AU  - Atkinson S
FAU - Butler, G
AU  - Butler G
FAU - Messent, A
AU  - Messent A
FAU - Gavrilovic, J
AU  - Gavrilovic J
FAU - Smith, B
AU  - Smith B
FAU - Timpl, R
AU  - Timpl R
FAU - Zardi, L
AU  - Zardi L
FAU - Murphy, G
AU  - Murphy G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Aggrecans)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Fibronectins)
RN  - 0 (Heparan Sulfate Proteoglycans)
RN  - 0 (Interleukin-1)
RN  - 0 (Laminin)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Proteoglycans)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tenascin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (nidogen)
RN  - 143972-95-6 (perlecan)
RN  - 9007-34-5 (Collagen)
RN  - 9050-30-0 (Heparitin Sulfate)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (Gelatinases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Aggrecans
MH  - Blotting, Western
MH  - Collagen/metabolism
MH  - Collagenases/chemistry/*metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme Activation/physiology
MH  - Escherichia coli/genetics
MH  - Extracellular Matrix/chemistry/metabolism
MH  - *Extracellular Matrix Proteins
MH  - Fibronectins/metabolism
MH  - Gelatinases/chemistry/*metabolism
MH  - *Heparan Sulfate Proteoglycans
MH  - Heparitin Sulfate/metabolism
MH  - Humans
MH  - Interleukin-1/metabolism
MH  - Laminin/metabolism
MH  - Lectins, C-Type
MH  - Matrix Metalloproteinase 1
MH  - Matrix Metalloproteinase 2
MH  - Membrane Glycoproteins/metabolism
MH  - Metalloendopeptidases/chemistry/*metabolism
MH  - Peptide Fragments/metabolism
MH  - Proteoglycans/metabolism
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Substrate Specificity
MH  - Tenascin/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 1998/02/14 00:00
MHDA- 1998/02/14 00:01
CRDT- 1998/02/14 00:00
PHST- 1998/02/14 00:00 [pubmed]
PHST- 1998/02/14 00:01 [medline]
PHST- 1998/02/14 00:00 [entrez]
AID - 10.1111/j.1432-1033.1997.00751.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1997 Dec 15;250(3):751-7. doi: 10.1111/j.1432-1033.1997.00751.x.