PMID- 9463351 OWN - NLM STAT- MEDLINE DCOM- 19980501 LR - 20220215 IS - 0950-1991 (Print) IS - 0950-1991 (Linking) VI - 125 IP - 6 DP - 1998 Mar TI - Systematic gain-of-function genetics in Drosophila. PG - 1049-57 AB - A modular misexpression system was used to carry out systematic gain-of-function genetic screens in Drosophila. The system is based on inducible expression of genes tagged by insertion of a P-element vector carrying a GAL4-regulated promoter oriented to transcribe flanking genomic sequences. To identify genes involved in eye and wing development, the 2300 independent lines were screened for dominant phenotypes. Among many novel genes, the screen identified known genes, including hedgehog and decapentaplegic, implicated in these processes. A genetic interaction screen for suppressors of a cell migration defect in a hypomorphic slow border cells mutant identified known genes with likely roles in tyrosine kinase signaling and control of actin cytoskeleton, among many novel genes. These studies demonstrate the ability of the modular misexpression system to identify developmentally important genes and suggest that it will be generally useful for genetic interaction screens. FAU - Rorth, P AU - Rorth P AD - Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA. rorth@mail1.ciwemb.edu FAU - Szabo, K AU - Szabo K FAU - Bailey, A AU - Bailey A FAU - Laverty, T AU - Laverty T FAU - Rehm, J AU - Rehm J FAU - Rubin, G M AU - Rubin GM FAU - Weigmann, K AU - Weigmann K FAU - Milan, M AU - Milan M FAU - Benes, V AU - Benes V FAU - Ansorge, W AU - Ansorge W FAU - Cohen, S M AU - Cohen SM LA - eng GR - GM53501/GM/NIGMS NIH HHS/United States GR - HG00750/HG/NHGRI NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - England TA - Development JT - Development (Cambridge, England) JID - 8701744 RN - 0 (CCAAT-Enhancer-Binding Proteins) RN - 0 (DNA-Binding Proteins) RN - 0 (Drosophila Proteins) RN - 0 (GAL4 protein, S cerevisiae) RN - 0 (Saccharomyces cerevisiae Proteins) RN - 0 (Transcription Factors) RN - 148997-45-9 (slbo protein, Drosophila) SB - IM MH - Animals MH - *CCAAT-Enhancer-Binding Proteins MH - Cell Movement/genetics MH - DNA-Binding Proteins MH - Drosophila/*genetics/growth & development MH - *Drosophila Proteins MH - Eye/growth & development MH - Female MH - Gene Expression Regulation, Developmental MH - Genes, Dominant MH - *Genes, Insect MH - Genome MH - Male MH - Mutation MH - Phenotype MH - *Saccharomyces cerevisiae Proteins MH - Suppression, Genetic MH - Transcription Factors/genetics MH - Wings, Animal/growth & development EDAT- 1998/05/09 00:00 MHDA- 1998/05/09 00:01 CRDT- 1998/05/09 00:00 PHST- 1998/05/09 00:00 [pubmed] PHST- 1998/05/09 00:01 [medline] PHST- 1998/05/09 00:00 [entrez] AID - 10.1242/dev.125.6.1049 [doi] PST - ppublish SO - Development. 1998 Mar;125(6):1049-57. doi: 10.1242/dev.125.6.1049.