PMID- 9550724
OWN - NLM
STAT- MEDLINE
DCOM- 19980707
LR  - 20220215
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 125
IP  - 10
DP  - 1998 May
TI  - An entire functional mammary gland may comprise the progeny from a single cell.
PG  - 1921-30
AB  - Any epithelial portion of a normal mouse mammary gland can reproduce an entire 
      functional gland when transplanted into an epithelium-free mammary fat pad. Mouse 
      mammary hyperplasias and tumors are clonal dominant populations and probably 
      represent the progeny of a single transformed cell. Our study provides evidence 
      that single multipotent stem cells positioned throughout the mature fully 
      developed mammary gland have the capacity to produce sufficient differentiated 
      progeny to recapitulate an entire functional gland. Our evidence also 
      demonstrates that these stem cells are self-renewing and are found with 
      undiminished capacities in the newly regenerated gland. We have taken advantage 
      of an experimental model where mouse mammary tumor virus infects mammary 
      epithelial cells and inserts a deoxyribonucleic acid copy(ies) of its genome 
      during replication. The insertions occur randomly within the somatic genome. 
      CzechII mice have no endogenous nucleic acid sequence homology with mouse mammary 
      tumor virus; therefore all viral insertions may be detected by Southern analysis 
      provided a sufficient number of cells contain a specific insertional event. 
      Transplantation of random fragments of infected CzechII mammary gland produced 
      clonal-dominant epithelial populations in epithelium-free mammary fat pads. 
      Serial transplantation of pieces of the clonally derived outgrowths produced 
      second generation glands possessing the same viral insertion sites providing 
      evidence for self-renewal of the original stem cell. Limiting dilution studies 
      with cell cultures derived from third generation clonal outgrowths demonstrated 
      that three multipotent but distinct mammary epithelial progenitors were present 
      in clonally derived mammary epithelial populations. Estimation of the potential 
      number of multipotent epithelial cells that may be evolved from an individual 
      mammary-specific stem cell by self-renewal is in the order of 10(12)-10(13). 
      Therefore, one stem cell might easily account for the renewal of mammary 
      epithelium over several transplant generations.
FAU - Kordon, E C
AU  - Kordon EC
AD  - Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, 
      MD 20892, USA.
FAU - Smith, G H
AU  - Smith GH
LA  - eng
PT  - Journal Article
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Cell Division
MH  - DNA, Viral/analysis
MH  - Epithelial Cells
MH  - Female
MH  - Mammary Glands, Animal/*cytology/transplantation
MH  - Mammary Tumor Virus, Mouse
MH  - Mice
MH  - Proviruses
MH  - *Stem Cells
EDAT- 1998/06/18 00:00
MHDA- 1998/06/18 00:01
CRDT- 1998/06/18 00:00
PHST- 1998/06/18 00:00 [pubmed]
PHST- 1998/06/18 00:01 [medline]
PHST- 1998/06/18 00:00 [entrez]
AID - 10.1242/dev.125.10.1921 [doi]
PST - ppublish
SO  - Development. 1998 May;125(10):1921-30. doi: 10.1242/dev.125.10.1921.