PMID- 9576952
OWN - NLM
STAT- MEDLINE
DCOM- 19980619
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 95
IP  - 10
DP  - 1998 May 12
TI  - Chromosomal instability in the descendants of unirradiated surviving cells after 
      alpha-particle irradiation.
PG  - 5730-3
AB  - We have demonstrated chromosomal instability in the clonal descendants of 
      hemopoietic stem cells after irradiating murine bone marrow with alpha-particles. 
      However, because cells that are irradiated by alpha-particles are defined by a 
      Poisson distribution of individual particle traversals, there is an inevitable 
      proportion of unirradiated cells in the surviving population. The calculated 
      expected proportions of irradiated and nonirradiated cells indicate that the 
      number of clonogenic cells transmitting chromosomal instability is greater than 
      the number expected to be hit and survive. To investigate further this 
      discrepancy, we studied the effects of interposing a grid between the cells and 
      the alpha-particle source so that the surviving population consists predominantly 
      of untraversed stem cells. Comparison with the same irradiation conditions 
      without the grid reveals that the same level of instability is induced. The data 
      confirm that alpha-particles induce chromosomal instability but instability is 
      demonstrated in the progeny of nonirradiated stem cells and must be due to 
      unexpected interactions between irradiated and nonirradiated cells. This 
      untargeted effect has important implications for mechanistic studies of radiation 
      action and for assessment of radiation risk.
FAU - Lorimore, S A
AU  - Lorimore SA
AD  - Radiation and Genome Stability Unit, Medical Research Council, Harwell, 
      Oxfordshire OX11 0RD, United Kingdom.
FAU - Kadhim, M A
AU  - Kadhim MA
FAU - Pocock, D A
AU  - Pocock DA
FAU - Papworth, D
AU  - Papworth D
FAU - Stevens, D L
AU  - Stevens DL
FAU - Goodhead, D T
AU  - Goodhead DT
FAU - Wright, E G
AU  - Wright EG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
SB  - IM
MH  - Abnormalities, Radiation-Induced/*genetics
MH  - Alpha Particles/*adverse effects
MH  - Animals
MH  - Chromosomes/*radiation effects
MH  - Clone Cells/radiation effects
MH  - Dose-Response Relationship, Radiation
MH  - Hematopoietic Stem Cells/radiation effects
MH  - Male
MH  - Mice
PMC - PMC20447
EDAT- 1998/05/20 00:00
MHDA- 1998/05/20 00:01
PMCR- 1998/11/12
CRDT- 1998/05/20 00:00
PHST- 1998/05/20 00:00 [pubmed]
PHST- 1998/05/20 00:01 [medline]
PHST- 1998/05/20 00:00 [entrez]
PHST- 1998/11/12 00:00 [pmc-release]
AID - 0788 [pii]
AID - 10.1073/pnas.95.10.5730 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1998 May 12;95(10):5730-3. doi: 
      10.1073/pnas.95.10.5730.