PMID- 9584136
OWN - NLM
STAT- MEDLINE
DCOM- 19980609
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 187
IP  - 10
DP  - 1998 May 18
TI  - Long-term persistence of activated cytotoxic T lymphocytes after viral infection 
      of the central nervous system.
PG  - 1575-82
AB  - Mice intranasally inoculated with influenza A/X-31 are protected against a 
      subsequent intracerebral challenge with the neurovirulent influenza A/WSN and 
      this heterotypic protection is mediated by CD8(+) cytotoxic T lymphocytes. We 
      have studied the kinetics of this secondary immune response and found that 
      despite the elimination of replication-competent virus by day 10, we were able to 
      recover activated influenza-specific cytotoxic T lymphocytes (CTLs) that killed 
      freshly ex vivo from the brains of mice for at least 320 d after the 
      intracerebral inoculation. The activated antiviral CTLs expressed high levels of 
      the early activation marker CD69, suggesting continuing TCR signaling despite a 
      lack of viral protein and major histocompatibility complex staining by 
      immunohistochemistry in the brain parenchyma and barely detectable levels of 
      viral nucleic acid by single and two-step reverse transcription PCR. Local 
      persistence of activated lymphocytes may be important for efficient long-term 
      responses to viruses prone to recrudesce in sites of relative immune privilege.
FAU - Hawke, S
AU  - Hawke S
AD  - Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, 
      Headington, Oxford OX3 9DU, United Kingdom. s.hawke@cxwms.ac.uk
FAU - Stevenson, P G
AU  - Stevenson PG
FAU - Freeman, S
AU  - Freeman S
FAU - Bangham, C R
AU  - Bangham CR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
SB  - IM
MH  - Animals
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Central Nervous System/*immunology/virology
MH  - *Cytotoxicity, Immunologic
MH  - *Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Orthomyxoviridae
MH  - Orthomyxoviridae Infections/*immunology
MH  - Time Factors
PMC - PMC2212297
EDAT- 1998/06/13 00:00
MHDA- 1998/06/13 00:01
PMCR- 1998/11/18
CRDT- 1998/06/13 00:00
PHST- 1998/06/13 00:00 [pubmed]
PHST- 1998/06/13 00:01 [medline]
PHST- 1998/06/13 00:00 [entrez]
PHST- 1998/11/18 00:00 [pmc-release]
AID - 10.1084/jem.187.10.1575 [doi]
PST - ppublish
SO  - J Exp Med. 1998 May 18;187(10):1575-82. doi: 10.1084/jem.187.10.1575.