PMID- 9590212
OWN - NLM
STAT- MEDLINE
DCOM- 19980528
LR  - 20081121
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 160
IP  - 10
DP  - 1998 May 15
TI  - Microglia are more efficient than astrocytes in antigen processing and in Th1 but 
      not Th2 cell activation.
PG  - 4671-80
AB  - Microglia and astrocytes, two glial cell populations of the central nervous 
      system, present Ag and stimulate T cell proliferation, but it is unclear whether 
      they preferentially activate Th1 or Th2 responses. We have investigated the 
      efficiency of microglia and astrocytes in the presentation of OVA peptide 323-339 
      or native OVA to Th1 and Th2 cell lines from DO11.10 TCR transgenic mice. Upon 
      stimulation with IFN-gamma, microglia express MHC class II molecules, CD40, and 
      ICAM-1 and efficiently present OVA 323-339, leading to T cell proliferation and 
      production of IL-2 and IFN-gamma by Th1 and of IL-4 by Th2 cells. 
      IFN-gamma-treated astrocytes, which express MHC class II and ICAM-1, present OVA 
      323-339 less efficiently to Th1 cells but are as efficient as microglia in 
      inducing IL-4 secretion by Th2 cells. However, astrocytes are much less potent 
      than microglia in presenting naturally processed OVA peptide to either T cell 
      subset, indicating inefficient Ag processing. The capacity of astrocytes and 
      microglia to stimulate Th1 and Th2 cells depends on their MHC class II expression 
      and does not involve ICAM-1, B7-1, or B7-2 molecules. However, CD40-CD40L 
      interactions contribute to Th1 activation by microglia. These data suggest that 
      microglia may play a role in the activation of Th1 and Th2 cells, whereas 
      astrocytes would restimulate mainly Th2 responses in the presence of appropriate 
      peptides. This differential capacity of brain APC to restimulate Th1 and Th2 
      responses may contribute to the reactivation and regulation of local inflammatory 
      processes during infectious and autoimmune diseases.
FAU - Aloisi, F
AU  - Aloisi F
AD  - Laboratory of Organ and System Pathophysiology, Istituto Superiore di Sanita, 
      Rome, Italy.
FAU - Ria, F
AU  - Ria F
FAU - Penna, G
AU  - Penna G
FAU - Adorini, L
AU  - Adorini L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - *Antigen Presentation
MH  - Astrocytes/*physiology
MH  - Cells, Cultured
MH  - Histocompatibility Antigens Class II/analysis
MH  - Intercellular Adhesion Molecule-1/analysis
MH  - Interferon-gamma/pharmacology
MH  - *Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microglia/*physiology
MH  - Ovalbumin/immunology
MH  - Th1 Cells/*immunology
MH  - Th2 Cells/*immunology
EDAT- 1998/05/20 00:00
MHDA- 1998/05/20 00:01
CRDT- 1998/05/20 00:00
PHST- 1998/05/20 00:00 [pubmed]
PHST- 1998/05/20 00:01 [medline]
PHST- 1998/05/20 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1998 May 15;160(10):4671-80.