PMID- 9603339
OWN - NLM
STAT- MEDLINE
DCOM- 19980615
LR  - 20191210
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 79 ( Pt 5)
DP  - 1998 May
TI  - Disruption of PML-associated nuclear bodies mediated by the human cytomegalovirus 
      major immediate early gene product.
PG  - 1233-45
AB  - The PML gene product is associated with a defined nuclear structure (10-20 per 
      cell) known variously as PML-bodies, ND10, PODs or Kr bodies. Certain conditions 
      are known to compromise the integrity of PML-bodies; these include environmental 
      stress (e.g. heat shock), a chromosomal translocation-associated acute 
      promyelocytic leukaemia, and infection with certain viruses [including human 
      cytomegalovirus (HCMV), herpes simplex virus type 1 and adenovirus]. Expression 
      of the HCMV major immediate early (IE) protein (IE1(491aa)) is by itself 
      sufficient to cause disruption of PML-bodies, resulting in the dispersal of the 
      PML antigen uniformly throughout the nucleus. In uninfected cells undergoing 
      mitosis PML is excluded from chromatin. However, both IE1(491aa) and PML were 
      observed to associate with mitotic chromosomes in cells infected with HCMV or 
      transfected with the IE1 gene. A series of in-frame IE1 deletion mutants was used 
      in DNA transfection experiments to identify two large sequence elements (aa 
      132-274 and the C-terminal aa 347-491) not required for dispersal of the PML 
      antigen. However, a putative leucine-zipper domain (aa 105-139), a putative 
      zinc-finger domain (aa 267-286) and exon 2 and 3 coding sequences (aa 6-85) were 
      required. The association of the IE1 gene product with chromatin required an 
      acidic domain near the C terminus (aa 421-486). The interaction of IE1(491aa) 
      with chromatin was therefore not required for the disruption of PML-bodies. Exon 
      2 (aa 1-24) was shown to encode a nuclear localization signal.
FAU - Wilkinson, G W
AU  - Wilkinson GW
AD  - Department of Medicine, University of Wales College of Medicine, Cardiff, UK. 
      WMDGWW@CARDIFF.AC.UK
FAU - Kelly, C
AU  - Kelly C
FAU - Sinclair, J H
AU  - Sinclair JH
FAU - Rickards, C
AU  - Rickards C
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Chromatin)
RN  - 0 (IE1 protein, cytomegalovirus)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Promyelocytic Leukemia Protein)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (Viral Proteins)
RN  - 143220-95-5 (PML protein, human)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Chromatin
MH  - Cytomegalovirus/*metabolism
MH  - Exons
MH  - Gene Deletion
MH  - Humans
MH  - Immediate-Early Proteins/genetics/*metabolism
MH  - Neoplasm Proteins/*metabolism
MH  - Nuclear Localization Signals
MH  - Nuclear Proteins/*metabolism
MH  - Promyelocytic Leukemia Protein
MH  - Transcription Factors/*metabolism
MH  - Tumor Suppressor Proteins
MH  - *Viral Proteins
EDAT- 1998/05/29 00:00
MHDA- 1998/05/29 00:01
CRDT- 1998/05/29 00:00
PHST- 1998/05/29 00:00 [pubmed]
PHST- 1998/05/29 00:01 [medline]
PHST- 1998/05/29 00:00 [entrez]
AID - 10.1099/0022-1317-79-5-1233 [doi]
PST - ppublish
SO  - J Gen Virol. 1998 May;79 ( Pt 5):1233-45. doi: 10.1099/0022-1317-79-5-1233.