PMID- 9798907
OWN - NLM
STAT- MEDLINE
DCOM- 19981130
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 71
IP  - 5
DP  - 1998 Nov
TI  - Interleukin-6-mediated autocrine growth promotion in human glioblastoma 
      multiforme cell line U87MG.
PG  - 1837-45
AB  - Human glioblastoma multiforme cell lines, brain tumor biopsy tissue, and normal 
      human fetal brain synthesize interleukin (IL)-6 and IL-6 receptor (IL-6R). 
      Neither of these is expressed in human neurons or neuroblastoma cell lines in 
      culture. Astrocytes from fetal brain grown in culture retain the ability to 
      synthesize IL-6 but do not express IL-6R as inferred from RT-PCR and Southern 
      blot studies. Coexpression of IL-6 and IL-6R in the glioblastoma cell line U87MG 
      is confirmed by immunofluorescence staining. Both specific monoclonal antibodies 
      against IL-6 and IL-6R and antisense oligonucleotide to IL-6 mRNA inhibit the 
      growth of U87MG cells in culture, suggesting the existence of a functional 
      autocrine growth loop. Anti-IL-6 antibodies also inhibit the growth of 
      glioblastoma cell lines U373 and U118. The expression of IL-6 by human fetal 
      astrocytes in culture is highly suggestive of its role as an oncofetal protein 
      responsible for rapid proliferation of fetal and tumor cells but not cells of 
      adult brain.
FAU - Goswami, S
AU  - Goswami S
AD  - Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
FAU - Gupta, A
AU  - Gupta A
FAU - Sharma, S K
AU  - Sharma SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Antibodies)
RN  - 0 (Antisense Elements (Genetics))
RN  - 0 (Culture Media)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin-6)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Antisense Elements (Genetics)/pharmacology
MH  - Autocrine Communication/*physiology
MH  - Brain/embryology/metabolism
MH  - Cell Division/drug effects/physiology
MH  - Culture Media/metabolism
MH  - Fetus/cytology/metabolism
MH  - Glioblastoma/*pathology/physiopathology
MH  - Humans
MH  - Interleukin-6/genetics/immunology/*physiology
MH  - Mice
MH  - Mice, Nude
MH  - Neuroglia/metabolism
MH  - Neurons/metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptors, Interleukin-6/genetics/immunology/metabolism
MH  - Tissue Distribution
MH  - Tumor Cells, Cultured/metabolism
EDAT- 1998/11/03 00:00
MHDA- 1998/11/03 00:01
CRDT- 1998/11/03 00:00
PHST- 1998/11/03 00:00 [pubmed]
PHST- 1998/11/03 00:01 [medline]
PHST- 1998/11/03 00:00 [entrez]
AID - 10.1046/j.1471-4159.1998.71051837.x [doi]
PST - ppublish
SO  - J Neurochem. 1998 Nov;71(5):1837-45. doi: 10.1046/j.1471-4159.1998.71051837.x.