PMID- 9858771
OWN - NLM
STAT- MEDLINE
DCOM- 19990303
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 223
IP  - 1-2
DP  - 1998 Nov 26
TI  - Amplification of erbB-4 oncogene occurs less frequently than that of erbB-2 in 
      primary human breast cancer.
PG  - 375-80
AB  - ErbB-4 protein is a recently discovered member of the ErbB family. The role of 
      ErbB-4 protein in mammary-gland tissue has not been definitively established. To 
      date, the expression of erbB-4 in breast tissue has been determined in only a few 
      cases and, to the best of our knowledge, its amplification has not been examined. 
      We therefore used the double differential polymerase chain reaction (ddPCR) for 
      determination of the amplification profile of erbB-4 and erbB-2, another gene 
      from the ErbB family, in human primary breast cancer specimens. We examined the 
      amplification of the genes in 20 normal breasts and 176 invasive breast cancer 
      samples. Amplification of erbB-2 was detected in 19% and erbB-4 in 13% of the 
      samples studied. Co-amplification of the two oncogenes was found in only five out 
      of 176 samples. Human breast cancer-derived cell lines in most cases overexpress 
      both erbB-2 and erbB-4 (Beerli et al., 1995. Mol. Cell Biol. 15, 6496-6505; Han 
      et al., 1995. Proc. Natl. Acad. Sci. USA 92, 9747-9751), but data on separate 
      erbB-2 overexpression, without overexpression of erbB-4, were also reported 
      (Wosikowski et al., 1997. Clin. Cancer Res. 3, 2405-2414). At the gene level, we 
      found that co-amplification of the genes in the case of human breast cancer is 
      rare. Moreover, an inverse association of the erbB-4 amplification with estrogen 
      receptor activity and direct correlation with the tumor size were found. Due to 
      these correlations, erbB-4 oncogene amplification can be assumed to be of 
      prognostic or predictive value in the diagnosis of breast cancer.
FAU - Vogt, U
AU  - Vogt U
AD  - European Laboratory Association Section Ibbenburen, Roggenkampstr. 10, 49477, 
      Ibbenburen, Germany.druvogt@aol.com
FAU - Bielawski, K
AU  - Bielawski K
FAU - Schlotter, C M
AU  - Schlotter CM
FAU - Bosse, U
AU  - Bosse U
FAU - Falkiewicz, B
AU  - Falkiewicz B
FAU - Podhajska, A J
AU  - Podhajska AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - EC 2.7.10.1 (ERBB4 protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
RN  - EC 2.7.10.1 (Receptor, ErbB-4)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Breast Neoplasms/*genetics/pathology
MH  - ErbB Receptors/*genetics
MH  - Female
MH  - *Gene Amplification
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Receptor, ErbB-2/*genetics
MH  - Receptor, ErbB-4
EDAT- 1998/12/22 00:00
MHDA- 1998/12/22 00:01
CRDT- 1998/12/22 00:00
PHST- 1998/12/22 00:00 [pubmed]
PHST- 1998/12/22 00:01 [medline]
PHST- 1998/12/22 00:00 [entrez]
AID - S0378-1119(98)00454-5 [pii]
AID - 10.1016/s0378-1119(98)00454-5 [doi]
PST - ppublish
SO  - Gene. 1998 Nov 26;223(1-2):375-80. doi: 10.1016/s0378-1119(98)00454-5.