PMID- 9859993
OWN - NLM
STAT- MEDLINE
DCOM- 19990107
LR  - 20220408
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 396
IP  - 6711
DP  - 1998 Dec 10
TI  - Control of apoptosis and mitotic spindle checkpoint by survivin.
PG  - 580-4
AB  - Progression of the cell cycle and control of apoptosis (programmed cell death) 
      are thought to be intimately linked processes, acting to preserve homeostasis and 
      developmental morphogenesis. Although proteins that regulate apoptosis have been 
      implicated in restraining cell-cycle entry and controlling ploidy (chromosome 
      number), the effector molecules at the interface between cell proliferation and 
      cell survival have remained elusive. Here we show that a new inhibitor of 
      apoptosis (IAP) protein, survivin, is expressed in the G2/M phase of the cell 
      cycle in a cycle-regulated manner. At the beginning of mitosis, survivin 
      associates with microtubules of the mitotic spindle in a specific and saturable 
      reaction that is regulated by microtubule dynamics. Disruption of 
      survivin-microtubule interactions results in loss of survivin's anti-apoptosis 
      function and increased caspase-3 activity, a mechanism involved in cell death, 
      during mitosis. These results indicate that survivin may counteract a default 
      induction of apoptosis in G2/M phase. The overexpression of survivin in cancer 
      may overcome this apoptotic checkpoint and favour aberrant progression of 
      transformed cells through mitosis.
FAU - Li, F
AU  - Li F
AD  - Boyer Center for Molecular Medicine, Department of Pathology, Yale University 
      School of Medicine, New Haven, Connecticut 06536, USA.
FAU - Ambrosini, G
AU  - Ambrosini G
FAU - Chu, E Y
AU  - Chu EY
FAU - Plescia, J
AU  - Plescia J
FAU - Tognin, S
AU  - Tognin S
FAU - Marchisio, P C
AU  - Marchisio PC
FAU - Altieri, D C
AU  - Altieri DC
LA  - eng
GR  - 496/TI_/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (BIRC5 protein, human)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proteins)
RN  - 0 (Survivin)
RN  - 0 (Tubulin)
RN  - 0 (Viral Proteins)
RN  - 0 (inhibitor of apoptosis, Nucleopolyhedrovirus)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Cell Cycle/physiology
MH  - G2 Phase/physiology
MH  - HeLa Cells
MH  - Humans
MH  - Inhibitor of Apoptosis Proteins
MH  - Mice
MH  - *Microtubule-Associated Proteins
MH  - Microtubules/physiology
MH  - Mitosis/physiology
MH  - Mutation
MH  - Neoplasm Proteins
MH  - Proteins/*physiology
MH  - Spindle Apparatus/*physiology
MH  - Survivin
MH  - Tubulin/physiology
MH  - Viral Proteins/genetics/physiology
EDAT- 1998/12/22 03:04
MHDA- 2001/03/23 10:01
CRDT- 1998/12/22 03:04
PHST- 1998/12/22 03:04 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1998/12/22 03:04 [entrez]
AID - 10.1038/25141 [doi]
PST - ppublish
SO  - Nature. 1998 Dec 10;396(6711):580-4. doi: 10.1038/25141.