PMID- 9888665
OWN - NLM
STAT- MEDLINE
DCOM- 19990315
LR  - 20190905
IS  - 0885-3177 (Print)
IS  - 0885-3177 (Linking)
VI  - 18
IP  - 1
DP  - 1999 Jan
TI  - Angiogenesis, angiogenic growth factors, and cell adhesion molecules are 
      upregulated in chronic pancreatic diseases: angiogenesis in chronic pancreatitis 
      and in pancreatic cancer.
PG  - 96-103
AB  - Recent epidemiologic evidence suggests that patients with chronic pancreatitis 
      (CP) have an increased risk of developing pancreatic carcinoma (PCA). In spite of 
      numerous similarities in both diseases, mechanisms for progression from CP to PCA 
      are poorly understood. We hypothesized that enhanced angiogenesis might play a 
      pivotal role in the etiology and histopathology of both CP and PCA, and thus form 
      a possible link between precancer and carcinoma. In surgical specimens of 18 
      patients with CP, 10 with PCA, and 18 controls, absolute numbers of blood vessels 
      and relative blood vessel density were assessed after immunostaining of 
      endothelial cells for von Willebrand factor and PECAM-1 (platelet/ endothelial 
      cell adhesion molecule-1). Furthermore, the expression of cell adhesion molecules 
      ICAM-1 (intercellular adhesion molecule-1) and VCAM-1 (vascular cell adhesion 
      molecule-1) and of VEGF (vascular endothelial growth factor) was investigated in 
      all specimens. Both CP and PCA exhibited areas of high vascular density ("hot 
      spots"). The mean number of blood vessels in these areas in PCA was 132.2+/-16.8 
      per mm2, and in CP, 99.2+/-7.4 per mm2. The mean vessel count in controls was 
      25.1+/-5.1. Relative vessel density was increased in both PCA (41.3+/-3.5%) and 
      CP (30.6+/-2.6%) versus controls (8.0+/-0.8%). Both absolute vessel count and 
      relative vessel density were significantly higher (p<0.05) in PCA than in CP. 
      Enhanced expression of ICAM-1 in CP and PCA was seen in ductal cells in CP and 
      cancer cells. In controls, ICAM-1 and VCAM-1 were expressed only at low levels in 
      endothelial cells. VCAM-1 was strongly expressed in acinar cells as well as in 
      ductal cells. In CP and PCA, VEGF was strongly expressed in ductal cells in CP as 
      well as in cancer cells. We show for the first time that angiogenic activity is 
      increased in both CP and PCA. Based on this study, we suggest that 
      antiangiogenesis might be a novel target for prevention or therapy in chronic 
      pancreatic diseases.
FAU - Kuehn, R
AU  - Kuehn R
AD  - Department of Surgery, University Hospital Eppendorf, University of Hamburg, 
      Germany.
FAU - Lelkes, P I
AU  - Lelkes PI
FAU - Bloechle, C
AU  - Bloechle C
FAU - Niendorf, A
AU  - Niendorf A
FAU - Izbicki, J R
AU  - Izbicki JR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Pancreas
JT  - Pancreas
JID - 8608542
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Lymphokines)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - 0 (von Willebrand Factor)
SB  - IM
MH  - Adenocarcinoma/blood supply/genetics/metabolism/*pathology
MH  - Cell Adhesion Molecules/*analysis/genetics
MH  - Chronic Disease
MH  - Endothelial Growth Factors/*analysis/genetics
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Lymphatic Metastasis
MH  - Lymphokines/*analysis/genetics
MH  - Neovascularization, Pathologic/*pathology
MH  - Pancreatic Neoplasms/blood supply/genetics/metabolism/*pathology
MH  - Pancreatitis/genetics/metabolism/*pathology
MH  - Platelet Endothelial Cell Adhesion Molecule-1/analysis/genetics
MH  - Vascular Cell Adhesion Molecule-1/analysis/genetics
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
MH  - von Willebrand Factor/analysis
EDAT- 1999/01/15 00:00
MHDA- 1999/01/15 00:01
CRDT- 1999/01/15 00:00
PHST- 1999/01/15 00:00 [pubmed]
PHST- 1999/01/15 00:01 [medline]
PHST- 1999/01/15 00:00 [entrez]
AID - 10.1097/00006676-199901000-00012 [doi]
PST - ppublish
SO  - Pancreas. 1999 Jan;18(1):96-103. doi: 10.1097/00006676-199901000-00012.