P02763 B7ZKQ5 Q5T539 Q5U067 Q8TC16 A1AG1_HUMAN Alpha-1-acid glycoprotein 1 AGP 1 Orosomucoid-1 OMD 1 ORM1 AGP1 Homo sapiens Human Eukaryota Metazoa Chordata Craniata Vertebrata Euteleostomi Mammalia Eutheria Euarchontoglires Primates Haplorrhini Catarrhini Hominidae Homo Structure of the human alpha 1-acid glycoprotein gene: sequence homology with other human acute phase protein genes. NUCLEOTIDE SEQUENCE [MRNA] VARIANT GLN-38 Molecular cloning and nucleotide sequence of human alpha 1 acid glycoprotein cDNA. NUCLEOTIDE SEQUENCE [MRNA] VARIANT GLN-38 Structure and expression of the genes coding for human alpha 1-acid glycoprotein. NUCLEOTIDE SEQUENCE [GENOMIC DNA] VARIANT GLN-38 Cloning of human full-length CDSs in BD Creator(TM) system donor vector. NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] VARIANT GLN-38 Complete sequencing and characterization of 21,243 full-length human cDNAs. NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] VARIANT GLN-38 Liver DNA sequence and analysis of human chromosome 9. NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] VARIANTS CYS-167 AND MET-174 Liver Structure of alpha 1-acid glycoprotein. The complete amino acid sequence, multiple amino acid substitutions, and homology with the immunoglobulins. PROTEIN SEQUENCE OF 19-129 PYROGLUTAMATE FORMATION AT GLN-19 Isolation and partial characterization of the cyanogen bromide fragments of alpha 1-acid glycoprotein and the elucidation of the amino acid sequence of the carboxyl-terminal cyanogen bromide fragment. PROTEIN SEQUENCE OF 129-201 The disulfide bonds of alpha1-acid glycoprotein. DISULFIDE BONDS Analysis of the five glycosylation sites of human alpha 1-acid glycoprotein. GLYCOSYLATION AT ASN-33; ASN-56; ASN-72; ASN-93 AND ASN-103 Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. GLYCOSYLATION AT ASN-33 A new strategy for identification of N-glycosylated proteins and unambiguous assignment of their glycosylation sites using HILIC enrichment and partial deglycosylation. GLYCOSYLATION AT ASN-33; ASN-72 AND ASN-93 IDENTIFICATION BY MASS SPECTROMETRY A proteomic analysis of human bile. GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33 AND ASN-93 Bile Screening for N-glycosylated proteins by liquid chromatography mass spectrometry. GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33; ASN-56; ASN-72; ASN-93 AND ASN-103 Plasma Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33; ASN-56; ASN-72; ASN-93 AND ASN-103 Plasma Selective binding of imatinib to the genetic variants of human alpha1-acid glycoprotein. FUNCTION Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry. GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-93 Saliva The drug binding site of human alpha1-acid glycoprotein: insight from induced circular dichroism and electronic absorption spectra. FUNCTION Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-93 AND ASN-103 Liver Enrichment of glycopeptides for glycan structure and attachment site identification. GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-33 AND ASN-72 STRUCTURE OF CARBOHYDRATES Cerebrospinal fluid Human urinary glycoproteomics; attachment site specific analysis of N- and O-linked glycosylations by CID and ECD. GLYCOSYLATION AT ASN-33 STRUCTURE OF CARBOHYDRATES IDENTIFICATION BY MASS SPECTROMETRY The 1.8-A crystal structure of alpha1-acid glycoprotein (Orosomucoid) solved by UV RIP reveals the broad drug-binding activity of this human plasma lipocalin. X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 20-201 DOMAIN DISULFIDE BONDS Human orosomucoid polymorphism: molecular basis of the three common ORM1 alleles, ORM1*F1, ORM1*F2, and ORM1*S. VARIANTS GLN-38 AND MET-174 Functions as a transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. P02763 P19652 false 4 P02763 P05121 false 4 Secreted Expressed by the liver and secreted in plasma. Synthesis is controlled by glucocorticoids, interleukin-1 and interleukin-6, It increases 5- to 50-fold upon inflammation. Contains a beta-barrel that binds various ligands in its interior. N-glycosylated. N-glycan heterogeneity at Asn-33: Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor). Three common alleles of ORM1 are known. ORM1*F1 has Gln-38/Val-174; ORM1*F2 has Gln-38/Met-174 and ORM1*S has Arg-38/Val-174. The sequence shown is that of allele ORM1*S. Belongs to the calycin superfamily. Lipocalin family. 3D-structure Acute phase Direct protein sequencing Disulfide bond Glycoprotein Proteomics identification Pyrrolidone carboxylic acid Reference proteome Secreted Signal Transport R Q R C V M K R MALSWVLTVLSLLPLLEAQIPLCANLVPVPITNATLDRITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQDQCIYNTTYLNVQRENGTISRYVGGQEHFAHLLILRDTKTYMLAFDVNDEKNWGLSVYADKPETTKEQLGEFYEALDCLRIPKSDVVYTDWKKDKCEPLEKQHEKERKQEEGES